Fast screening method for detection of acyl-HSL-degrading soil isolates

S. Jafra; J.M. van der Wolf

doi:10.1016/j.mimet.2004.01.015

Fast screening method for detection of acyl-HSL-degrading soil isolates

S. Jafra, J.M. van der Wolf

Research output: Contribution to journal › Article › Academic › peer-review

21 Citations (Scopus)

Abstract

A reliable method was developed for screening of bacteria isolates capable of degrading acyl-HSLs, the signal molecules in quorum-sensing-mediated processes of many Proteobacteria. The microtiter assay was based on the use of a GFP-marked Escherichia coli strain, which fluoresces upon the presence of acyl-HSLs. Measurement of GFP fluorescence with a Molecular Imager FX scanner (fluorometer) detected isolates capable of degrading acyl-HSLs. The potential of this method was demonstrated by isolation of different bacteria from a potato rhizosphere able to inactivate synthetic and natural acyl HSLs produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) (Erwinia carotovora subsp. carotovora (Ecc))

Original language	English
Pages (from-to)	415-420
Journal	Journal of Microbiological Methods
Volume	57
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2004.01.015
Publication status	Published - 2004

Keywords

n-acylhomoserine lactones
quorum-sensing signals
pseudomonas-aeruginosa pao1
plant-associated bacteria
agrobacterium-tumefaciens
variovorax-paradoxus
virulence
molecules
chromatography
expression

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10.1016/j.mimet.2004.01.015

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title = "Fast screening method for detection of acyl-HSL-degrading soil isolates",

abstract = "A reliable method was developed for screening of bacteria isolates capable of degrading acyl-HSLs, the signal molecules in quorum-sensing-mediated processes of many Proteobacteria. The microtiter assay was based on the use of a GFP-marked Escherichia coli strain, which fluoresces upon the presence of acyl-HSLs. Measurement of GFP fluorescence with a Molecular Imager FX scanner (fluorometer) detected isolates capable of degrading acyl-HSLs. The potential of this method was demonstrated by isolation of different bacteria from a potato rhizosphere able to inactivate synthetic and natural acyl HSLs produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) (Erwinia carotovora subsp. carotovora (Ecc))",

keywords = "n-acylhomoserine lactones, quorum-sensing signals, pseudomonas-aeruginosa pao1, plant-associated bacteria, agrobacterium-tumefaciens, variovorax-paradoxus, virulence, molecules, chromatography, expression",

author = "S. Jafra and {van der Wolf}, J.M.",

year = "2004",

doi = "10.1016/j.mimet.2004.01.015",

language = "English",

volume = "57",

pages = "415--420",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Fast screening method for detection of acyl-HSL-degrading soil isolates

AU - Jafra, S.

AU - van der Wolf, J.M.

PY - 2004

Y1 - 2004

N2 - A reliable method was developed for screening of bacteria isolates capable of degrading acyl-HSLs, the signal molecules in quorum-sensing-mediated processes of many Proteobacteria. The microtiter assay was based on the use of a GFP-marked Escherichia coli strain, which fluoresces upon the presence of acyl-HSLs. Measurement of GFP fluorescence with a Molecular Imager FX scanner (fluorometer) detected isolates capable of degrading acyl-HSLs. The potential of this method was demonstrated by isolation of different bacteria from a potato rhizosphere able to inactivate synthetic and natural acyl HSLs produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) (Erwinia carotovora subsp. carotovora (Ecc))

AB - A reliable method was developed for screening of bacteria isolates capable of degrading acyl-HSLs, the signal molecules in quorum-sensing-mediated processes of many Proteobacteria. The microtiter assay was based on the use of a GFP-marked Escherichia coli strain, which fluoresces upon the presence of acyl-HSLs. Measurement of GFP fluorescence with a Molecular Imager FX scanner (fluorometer) detected isolates capable of degrading acyl-HSLs. The potential of this method was demonstrated by isolation of different bacteria from a potato rhizosphere able to inactivate synthetic and natural acyl HSLs produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) (Erwinia carotovora subsp. carotovora (Ecc))

KW - n-acylhomoserine lactones

KW - quorum-sensing signals

KW - pseudomonas-aeruginosa pao1

KW - plant-associated bacteria

KW - agrobacterium-tumefaciens

KW - variovorax-paradoxus

KW - virulence

KW - molecules

KW - chromatography

KW - expression

U2 - 10.1016/j.mimet.2004.01.015

DO - 10.1016/j.mimet.2004.01.015

M3 - Article

SN - 0167-7012

VL - 57

SP - 415

EP - 420

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

Fast screening method for detection of acyl-HSL-degrading soil isolates

Abstract

Keywords

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