Fast screening method for detection of acyl-HSL-degrading soil isolates

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    Abstract

    A reliable method was developed for screening of bacteria isolates capable of degrading acyl-HSLs, the signal molecules in quorum-sensing-mediated processes of many Proteobacteria. The microtiter assay was based on the use of a GFP-marked Escherichia coli strain, which fluoresces upon the presence of acyl-HSLs. Measurement of GFP fluorescence with a Molecular Imager FX scanner (fluorometer) detected isolates capable of degrading acyl-HSLs. The potential of this method was demonstrated by isolation of different bacteria from a potato rhizosphere able to inactivate synthetic and natural acyl HSLs produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) (Erwinia carotovora subsp. carotovora (Ecc))
    Original languageEnglish
    Pages (from-to)415-420
    JournalJournal of Microbiological Methods
    Volume57
    Issue number3
    DOIs
    Publication statusPublished - 2004

    Keywords

    • n-acylhomoserine lactones
    • quorum-sensing signals
    • pseudomonas-aeruginosa pao1
    • plant-associated bacteria
    • agrobacterium-tumefaciens
    • variovorax-paradoxus
    • virulence
    • molecules
    • chromatography
    • expression

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