Fast determination of the degree of methyl esterification of pectins by head-space GC.

M.M.H. Huisman, A. Oosterveld, H.A. Schols

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)

Abstract

A new, fast method for the quantitative analysis of methoxyl groups in pectin using head-space gas chromatography (HS-GC) has been developed. With this method, results were obtained which were in reasonable agreement with the conventional HPLC method, and the reproducibility of the measurements is high. The advantages of the HS-GC method are that only a small amount of sample (2 mg) per analysis is needed, the chromatogram shows a nice symmetrically shaped methanol peak which is very easy to integrate, the sample preparation for HS-GC is short and easy, and for soluble pectins the sample in the head space vial can also directly be used for analysis of the galacturonic acid content and the degree of acetylation.
Original languageEnglish
Pages (from-to)665-668
JournalFood Hydrocolloids
Volume18
Issue number4
DOIs
Publication statusPublished - 2004

Keywords

  • ester content
  • methanol

Fingerprint Dive into the research topics of 'Fast determination of the degree of methyl esterification of pectins by head-space GC.'. Together they form a unique fingerprint.

Cite this