Fast chromatographic separation for the quantitation of the main flavone dyes in Reseda luteola (weld)

A. Villela, E.J.C. van der Klift, E.S.G.M. Mattheussens, G.C.H. Derksen, H. Zuilhof, T.A. van Beek

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In the past decades, there has been a renewed interest in the use of natural dye plants for textile dyeing, e.g. Reseda luteola (weld). Its main yellow dye constituents are the flavones luteolin-7,3'-O-diglucoside, luteolin-7-O-glucoside and luteolin. The aim of this work was to develop a simple validated industrially usable quantitative method to assess the flavone content of R. luteola samples. The flavones were overnight extracted from the dried and ground aerial parts of the plant at room temperature via maceration with methanol-water 8:2. Afterwards, they were quantified through internal standardisation against chrysin by RP-HPLC-UV at 345nm. The efficiency of the one-step extraction was 95%. The limits of detection (LOD) and quantitation (LOQ) were =1ng and =3ng, respectively, providing ample sensitivity for the purpose. The precision expressed as relative standard deviation of the entire method was
Original languageEnglish
Pages (from-to)8544-8550
JournalJournal of Chromatography. A, Including electrophoresis and other separation methods
Issue number47
Publication statusPublished - 2011



  • performance liquid-chromatography
  • diode-array detection
  • l.
  • identification
  • quantification
  • bilobalide
  • portugal

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