Fast biosensor immunoassays for the detection of cows' milk in the milk of ewes and goats

W. Haasnoot, N.G.E. Smits, A.E.M. Voncken, M.G.E.G. Bremer

    Research output: Contribution to journalArticleAcademicpeer-review

    39 Citations (Scopus)

    Abstract

    Two monoclonal antibodies (MAb) raised against bovine K-casein were developed and applied in an automated optical biosensor (Biacore 3000) to create easy and fast direct and inhibition biosensor immunoassays (BIA) for the detection of cows' milk in the milk of ewes and goats. With both assay formats, low limits of detection (350 tests); and the wide measurement range (0·1 to 10% cows' milk). Despite these advantages, the inhibition BIA (using K-casein immobilized on the chip) was preferred because of the possible application of non-purified Mab, the higher responses, the higher sensitivity at relevant low percentages of cows' milk and its robustness (>800 cycles per chip)
    Original languageEnglish
    Pages (from-to)322-329
    JournalJournal of Dairy Research
    Volume71
    Issue number3
    DOIs
    Publication statusPublished - 2004

    Keywords

    • linked-immunosorbent-assay
    • bovine beta-casein
    • monoclonal-antibodies
    • polyclonal antibodies
    • liquid-chromatography
    • k-casein
    • cheese
    • elisa
    • proteins
    • ovine

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