Fasciola hepatica juveniles express immunodominant cathepsin L proteins, which are mainly found in their immature, procathepsin form. A gene encoding such a procathepsin L (FheCL3) was expressed by a baculovirus recombinant and by Saccharomyces cerevisiae. The glycosylated FheCL3 proteins obtained by both systems were used in a vaccination/challenge experiment in rats. Both antigens evoked similar antibody responses, but only the baculovirus expressed FheCL3 caused a significant protection against the number of liver flukes (52% protection, P = 0.01), whereas the S. cerevisiae expressed FheCL3 did not. In a second experiment in rats, deglycosylated versions of both antigens were used, but this did not improve their efficacies.
- cathepsin-l proteinases
- functional expression
- liver fluke
Reszka, N., Cornelissen, J. B. W. J., Harmsen, M. M., de Bree, J., Boersma, W. J. A., & Rijsewijk, F. A. M. (2005). Fasciola hepatica procathepsin L3 protein expressed by a baculovirus recombinant can partly protect rats against fasciolosis. Vaccine, 23(23), 2987-2993. https://doi.org/10.1016/j.vaccine.2004.12.007