Fasciola hepatica procathepsin L3 protein expressed by a baculovirus recombinant can partly protect rats against fasciolosis

N. Reszka, J.B.W.J. Cornelissen, M.M. Harmsen, J. de Bree, W.J.A. Boersma, F.A.M. Rijsewijk

    Research output: Contribution to journalArticleAcademicpeer-review

    20 Citations (Scopus)

    Abstract

    Fasciola hepatica juveniles express immunodominant cathepsin L proteins, which are mainly found in their immature, procathepsin form. A gene encoding such a procathepsin L (FheCL3) was expressed by a baculovirus recombinant and by Saccharomyces cerevisiae. The glycosylated FheCL3 proteins obtained by both systems were used in a vaccination/challenge experiment in rats. Both antigens evoked similar antibody responses, but only the baculovirus expressed FheCL3 caused a significant protection against the number of liver flukes (52% protection, P = 0.01), whereas the S. cerevisiae expressed FheCL3 did not. In a second experiment in rats, deglycosylated versions of both antigens were used, but this did not improve their efficacies.
    Original languageEnglish
    Pages (from-to)2987-2993
    JournalVaccine
    Volume23
    Issue number23
    DOIs
    Publication statusPublished - 2005

    Keywords

    • cathepsin-l proteinases
    • saccharomyces-cerevisiae
    • functional expression
    • liver fluke
    • vaccines
    • sheep
    • identification
    • vaccination
    • ruminants
    • immunity

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