TY - JOUR
T1 - Factors influencing cDNA microarray hybridization on silylated glass slides
AU - van Franssen-Hal, N.L.W.
AU - Vorst, O.F.J.
AU - Kramer, E.H.M.
AU - Hall, R.D.
AU - Keijer, J.
PY - 2002
Y1 - 2002
N2 - cDNA microarray technology is becoming the technique of choice for studying gene expression and gene expression patterns. Although experimental protocols are available, only limited methodological information on microarray manufacture, hybridization, and signal interpretation has been published. The aim of this paper is to provide more insight into the practical aspects of microarray construction and hybridization. The influence of the size, composition, and concentration of the spotted DNA fragments on the final hybridization signal and the effect of hybridization volume, sample concentration, and sample depletion have been tested and are discussed
AB - cDNA microarray technology is becoming the technique of choice for studying gene expression and gene expression patterns. Although experimental protocols are available, only limited methodological information on microarray manufacture, hybridization, and signal interpretation has been published. The aim of this paper is to provide more insight into the practical aspects of microarray construction and hybridization. The influence of the size, composition, and concentration of the spotted DNA fragments on the final hybridization signal and the effect of hybridization volume, sample concentration, and sample depletion have been tested and are discussed
KW - gene-expression
KW - oligonucleotide arrays
KW - protein microarrays
KW - dna
U2 - 10.1016/S0003-2697(02)00203-8
DO - 10.1016/S0003-2697(02)00203-8
M3 - Article
SN - 0003-2697
VL - 308
SP - 5
EP - 17
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -