TY - JOUR
T1 - Facilitated delignification in CAD deficient transgenic poplar studied by confocal Raman spectroscopy imaging
AU - Segmehl, Jana S.
AU - Keplinger, Tobias
AU - Krasnobaev, Artem
AU - Berg, John K.
AU - Willa, Christoph
AU - Burgert, Ingo
PY - 2019/1/5
Y1 - 2019/1/5
N2 - Lignocellulosic biomass represents the only renewable carbon resource which is available in sufficient amounts to be considered as an alternative for our fossil-based carbon economy. However, an efficient biochemical conversion of lignocellulosic feedstocks is hindered by the natural recalcitrance of the biomass as a result of a dense network of cellulose, hemicelluloses, and lignin. These polymeric interconnections make a pretreatment of the biomass necessary in order to enhance the susceptibility of the polysaccharides. Here, we report on a detailed analysis of the favourable influence of genetic engineering for two common delignification protocols for lignocellulosic biomass, namely acidic bleaching and soda pulping, on the example of CAD deficient poplar. The altered lignin structure of the transgenic poplar results in a significantly accelerated and more complete lignin removal at lower temperatures and shorter reaction times compared to wildtype poplar. To monitor the induced chemical and structural alterations at the tissue level, confocal Raman spectroscopy imaging, FT-IR spectroscopy, and X-ray diffraction were used.
AB - Lignocellulosic biomass represents the only renewable carbon resource which is available in sufficient amounts to be considered as an alternative for our fossil-based carbon economy. However, an efficient biochemical conversion of lignocellulosic feedstocks is hindered by the natural recalcitrance of the biomass as a result of a dense network of cellulose, hemicelluloses, and lignin. These polymeric interconnections make a pretreatment of the biomass necessary in order to enhance the susceptibility of the polysaccharides. Here, we report on a detailed analysis of the favourable influence of genetic engineering for two common delignification protocols for lignocellulosic biomass, namely acidic bleaching and soda pulping, on the example of CAD deficient poplar. The altered lignin structure of the transgenic poplar results in a significantly accelerated and more complete lignin removal at lower temperatures and shorter reaction times compared to wildtype poplar. To monitor the induced chemical and structural alterations at the tissue level, confocal Raman spectroscopy imaging, FT-IR spectroscopy, and X-ray diffraction were used.
KW - CAD deficient poplar
KW - Cellulose conformational change
KW - Facilitated delignification
KW - Lignocellulosic biomass
KW - Raman spectroscopy imaging
KW - X-ray diffraction
U2 - 10.1016/j.saa.2018.07.080
DO - 10.1016/j.saa.2018.07.080
M3 - Article
AN - SCOPUS:85051136783
SN - 1386-1425
VL - 206
SP - 177
EP - 184
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
ER -