Facile functionalization of peptide nucleic acids (PNAs) for antisense and single nucleotide polymorphism detection

Digvijay Gahtory, Merita Murtola, Maarten M.J. Smulders, Tom Wennekes, Han Zuilhof*, Roger Strömberg, Bauke Albada

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

In this report, we show how a convenient on-resin copper-click functionalization of azido-functionalized peptide nucleic acids (PNAs) allows various PNA-based detection strategies. Firstly, a thiazole orange (TO) clicked PNA probe facilitates a binary readout when combined with F/Q labeled DNA, giving increased sensitivity for antisense detection. Secondly, our TO-PNA conjugate also allows single nucleotide polymorphism detection. Since antisense detection is also possible in the absence of the TO label, our sensing platform based on azido-d-ornithine containing PNA even allows for additional and more advanced functionalization and sensing strategies.

Original languageEnglish
Pages (from-to)6710-6714
JournalOrganic & Biomolecular Chemistry
Volume15
Issue number32
DOIs
Publication statusPublished - 2017

Fingerprint Dive into the research topics of 'Facile functionalization of peptide nucleic acids (PNAs) for antisense and single nucleotide polymorphism detection'. Together they form a unique fingerprint.

Cite this