Extraction of steviol glycosides from fresh Stevia using acidified water; comparison to hot water extraction, including purification

A.M.J. Kootstra, Sander Huurman

Research output: Book/ReportReportProfessional

Abstract

This report describes a practical comparison of an acidified water extraction of freshly harvested Stevia
plants (the NewFoss method) to the hot water extraction of dried Stevia plants, the industry standard. Both
extracts are subsequently purified using lab-/bench scale standard industrial technology: coagulation,
filtration, adsorption, and ion exchange.
The reason for this comparison is the low purity of steviol glycosides reached in tests where an acidified
water extract was purified using mainly ultrafiltration in 2014 and 2015. Therefore, the purification potential
of the acidified water extract while using standard industrial technology needed to be assessed, in turn in
order to show the potential for the acidified water extraction as a viable extraction technology for steviol
glycosides from Stevia.
The combination of coagulation, filtration, adsorption, and ion exchange results in a 80.6 wt% steviol
glycosides in the dry matter of the purified acidified water extraction. This is very close to the 85.4 wt%
reached when the same purification processes are performed on a hot water extract. Acidified water extract
of fresh Stevia can be purified using the same standard industrial technology, to a similarly high level as a
hot water extract of dried Stevia. This strengthens the potential for the acidified water extraction of steviol
glycosides from fresh Stevia.
Original languageEnglish
Place of PublicationLelystad
PublisherWageningen UR, PPO/Acrres
Number of pages27
Publication statusPublished - 2017

Publication series

NamePagv-nr.
No.722

Fingerprint

Glycosides
Purification
Water
Coagulation
Ion exchange
steviol
Adsorption
Ultrafiltration

Cite this

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abstract = "This report describes a practical comparison of an acidified water extraction of freshly harvested Steviaplants (the NewFoss method) to the hot water extraction of dried Stevia plants, the industry standard. Bothextracts are subsequently purified using lab-/bench scale standard industrial technology: coagulation,filtration, adsorption, and ion exchange.The reason for this comparison is the low purity of steviol glycosides reached in tests where an acidifiedwater extract was purified using mainly ultrafiltration in 2014 and 2015. Therefore, the purification potentialof the acidified water extract while using standard industrial technology needed to be assessed, in turn inorder to show the potential for the acidified water extraction as a viable extraction technology for steviolglycosides from Stevia.The combination of coagulation, filtration, adsorption, and ion exchange results in a 80.6 wt{\%} steviolglycosides in the dry matter of the purified acidified water extraction. This is very close to the 85.4 wt{\%}reached when the same purification processes are performed on a hot water extract. Acidified water extractof fresh Stevia can be purified using the same standard industrial technology, to a similarly high level as ahot water extract of dried Stevia. This strengthens the potential for the acidified water extraction of steviolglycosides from fresh Stevia.",
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Extraction of steviol glycosides from fresh Stevia using acidified water; comparison to hot water extraction, including purification. / Kootstra, A.M.J.; Huurman, Sander.

Lelystad : Wageningen UR, PPO/Acrres, 2017. 27 p. (Pagv-nr.; No. 722).

Research output: Book/ReportReportProfessional

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