Extraction of carotenoids from feces enabling the bioavailability of ß-carotene to be studied in Indonesian children

M. van Lieshout, C.E. West, P. van de Bovenkamp, Y. Wang, Y. Sun, R.B. van Breemen, D. Permaesih, Muhilal, M.A. Verhoeven, A.F.L. Creemers, J. Lugtenburg

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Previously, we have presented a method for quantifying beta-carotene bioavailability based on analysis in serum, following administration of C-13-labeled beta-carotene. Because stool samples can be collected noninvasively, we have now extended the method to measure the bioavailability based on measurements in feces. An extraction method was developed to enable measurement of concentrations and degree of isotopic enrichment of retinol, retinyl palmitate and carotenoids in feces. Relative bioavailability of beta-carotene from pumpkin (n = 6) was found to be 1.8 times (interval, 0.6, 5.5) greater than that from spinach (n = 8), based on data from feces compared with 1.7 times (interval, 0.9, 3.1) based on data from serum.
Original languageEnglish
Pages (from-to)5123-5130
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number17
DOIs
Publication statusPublished - 2003

Keywords

  • mass-spectrometry
  • retinol
  • humans
  • bioefficacy

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