Extraction of azadirachtin a from neem seed kernels by supercritical fluid and its evaluation by HPLC and LC/MS

Patrizia Ambrosino; Rosa Fresa; Vincenzo Fogliano; Simona M. Monti; Alberto Ritieni

doi:10.1021/jf9905368

Extraction of azadirachtin a from neem seed kernels by supercritical fluid and its evaluation by HPLC and LC/MS

Patrizia Ambrosino, Rosa Fresa, Vincenzo Fogliano, Simona M. Monti, Alberto Ritieni^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

42 Citations (Scopus)

Abstract

A new supercritical extraction methodology was applied to extract azadirachtin A (AZA-A) from neem seed kernels. Supercritical and liquid carbon dioxide (CO₂) were used as extractive agents in a three-separation- stage supercritical pilot plant. Subcritical conditions were tested too. Comparisons were carried out by calculating the efficiency of the pilot plant with respect to the milligrams per kilogram of seeds (ms/mo) of AZA-A extracted. The most convenient extraction was gained using an ms/mo ratio of 119 rather than 64. For supercritical extraction, a separation of cuticular waxes from oil was set up in the pilot plant. HPLC and electrospray mass spectroscopy were used to monitor the yield of AZA-A extraction.

Original language	English
Pages (from-to)	5252-5256
Number of pages	5
Journal	Journal of Agricultural and Food Chemistry
Volume	47
Issue number	12
DOIs	https://doi.org/10.1021/jf9905368
Publication status	Published - Dec 1999
Externally published	Yes

Keywords

Azadirachtin A
HPLC
LC/MS
Neem seed kernels
Supercritical fluid extraction

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10.1021/jf9905368

Cite this

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title = "Extraction of azadirachtin a from neem seed kernels by supercritical fluid and its evaluation by HPLC and LC/MS",

abstract = "A new supercritical extraction methodology was applied to extract azadirachtin A (AZA-A) from neem seed kernels. Supercritical and liquid carbon dioxide (CO2) were used as extractive agents in a three-separation- stage supercritical pilot plant. Subcritical conditions were tested too. Comparisons were carried out by calculating the efficiency of the pilot plant with respect to the milligrams per kilogram of seeds (ms/mo) of AZA-A extracted. The most convenient extraction was gained using an ms/mo ratio of 119 rather than 64. For supercritical extraction, a separation of cuticular waxes from oil was set up in the pilot plant. HPLC and electrospray mass spectroscopy were used to monitor the yield of AZA-A extraction.",

keywords = "Azadirachtin A, HPLC, LC/MS, Neem seed kernels, Supercritical fluid extraction",

author = "Patrizia Ambrosino and Rosa Fresa and Vincenzo Fogliano and Monti, {Simona M.} and Alberto Ritieni",

year = "1999",

month = dec,

doi = "10.1021/jf9905368",

language = "English",

volume = "47",

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journal = "Journal of Agricultural and Food Chemistry",

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publisher = "American Chemical Society",

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TY - JOUR

T1 - Extraction of azadirachtin a from neem seed kernels by supercritical fluid and its evaluation by HPLC and LC/MS

AU - Ambrosino, Patrizia

AU - Fresa, Rosa

AU - Fogliano, Vincenzo

AU - Monti, Simona M.

AU - Ritieni, Alberto

PY - 1999/12

Y1 - 1999/12

N2 - A new supercritical extraction methodology was applied to extract azadirachtin A (AZA-A) from neem seed kernels. Supercritical and liquid carbon dioxide (CO2) were used as extractive agents in a three-separation- stage supercritical pilot plant. Subcritical conditions were tested too. Comparisons were carried out by calculating the efficiency of the pilot plant with respect to the milligrams per kilogram of seeds (ms/mo) of AZA-A extracted. The most convenient extraction was gained using an ms/mo ratio of 119 rather than 64. For supercritical extraction, a separation of cuticular waxes from oil was set up in the pilot plant. HPLC and electrospray mass spectroscopy were used to monitor the yield of AZA-A extraction.

AB - A new supercritical extraction methodology was applied to extract azadirachtin A (AZA-A) from neem seed kernels. Supercritical and liquid carbon dioxide (CO2) were used as extractive agents in a three-separation- stage supercritical pilot plant. Subcritical conditions were tested too. Comparisons were carried out by calculating the efficiency of the pilot plant with respect to the milligrams per kilogram of seeds (ms/mo) of AZA-A extracted. The most convenient extraction was gained using an ms/mo ratio of 119 rather than 64. For supercritical extraction, a separation of cuticular waxes from oil was set up in the pilot plant. HPLC and electrospray mass spectroscopy were used to monitor the yield of AZA-A extraction.

KW - Azadirachtin A

KW - HPLC

KW - LC/MS

KW - Neem seed kernels

KW - Supercritical fluid extraction

U2 - 10.1021/jf9905368

DO - 10.1021/jf9905368

M3 - Article

C2 - 10606604

AN - SCOPUS:0033381442

SN - 0021-8561

VL - 47

SP - 5252

EP - 5256

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 12

ER -

Extraction of azadirachtin a from neem seed kernels by supercritical fluid and its evaluation by HPLC and LC/MS

Abstract

Keywords

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