Extraction of azadirachtin a from neem seed kernels by supercritical fluid and its evaluation by HPLC and LC/MS

Patrizia Ambrosino, Rosa Fresa, Vincenzo Fogliano, Simona M. Monti, Alberto Ritieni*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

34 Citations (Scopus)

Abstract

A new supercritical extraction methodology was applied to extract azadirachtin A (AZA-A) from neem seed kernels. Supercritical and liquid carbon dioxide (CO2) were used as extractive agents in a three-separation- stage supercritical pilot plant. Subcritical conditions were tested too. Comparisons were carried out by calculating the efficiency of the pilot plant with respect to the milligrams per kilogram of seeds (ms/mo) of AZA-A extracted. The most convenient extraction was gained using an ms/mo ratio of 119 rather than 64. For supercritical extraction, a separation of cuticular waxes from oil was set up in the pilot plant. HPLC and electrospray mass spectroscopy were used to monitor the yield of AZA-A extraction.

Original languageEnglish
Pages (from-to)5252-5256
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume47
Issue number12
DOIs
Publication statusPublished - Dec 1999
Externally publishedYes

Keywords

  • Azadirachtin A
  • HPLC
  • LC/MS
  • Neem seed kernels
  • Supercritical fluid extraction

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