Extensive chromosomal reshuffling drives evolution of virulence in an asexual pathogen

R. de Jonge, M. Bolton, A. Kombrink, K. Yadeta, G.C.M. van den Berg-Velthuis, B.P.H.J. Thomma

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Sexual recombination drives genetic diversity in eukaryotic genomes, and fosters adaptation to novel environmental challenges. Although strictly asexual microorganisms are often considered as evolutionary dead ends, they comprise many devastating plant pathogens. Presently, it remains unknown how such asexual pathogens generate the genetic variation that is required for quick adaptation and evolution in the arms race with their hosts. Here we show that extensive chromosomal rearrangements in the strictly asexual plant pathogenic fungus Verticillium dahliae establish highly dynamic ‘plastic’ genomic regions that act as a source for genetic variation to mediate aggressiveness. We show that these plastic regions are greatly enriched for in planta-expressed effector genes, encoding secreted proteins that enable host colonization including the previously identified race 1-specific effector Ave1 that activates Ve1-mediated resistance in tomato. The plastic regions occur at the flanks of chromosomal breakpoints and are enriched for repetitive sequence elements, especially retrotransposons. Our results demonstrate that asexual pathogens may evolve by prompting chromosomal rearrangements, enabling rapid development of novel effector genes. Likely, chromosomal reshuffling is a general mechanism for adaptation in asexually propagating organisms.
Original languageEnglish
Title of host publicationBook of Abstracts 27th Fungal Genetics Conference, Asilomar, Pacific Grove, California, USA, 12-17 March 2013
Pages182-183
Publication statusPublished - 2013
Event27th Fungal Genetics Conference -
Duration: 12 Mar 201317 Mar 2013

Conference

Conference27th Fungal Genetics Conference
Period12/03/1317/03/13

Fingerprint Dive into the research topics of 'Extensive chromosomal reshuffling drives evolution of virulence in an asexual pathogen'. Together they form a unique fingerprint.

  • Cite this

    de Jonge, R., Bolton, M., Kombrink, A., Yadeta, K., van den Berg-Velthuis, G. C. M., & Thomma, B. P. H. J. (2013). Extensive chromosomal reshuffling drives evolution of virulence in an asexual pathogen. In Book of Abstracts 27th Fungal Genetics Conference, Asilomar, Pacific Grove, California, USA, 12-17 March 2013 (pp. 182-183)