Extended DNA Fibers for High-Resolution Mapping

Paul Fransz*, José van de Belt, Hans de Jong

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

DNA fiber-FISH is an easy and simple light microscopic method to map unique and repeat sequences relative to each other at the molecular scale. A standard fluorescence microscope and a DNA labeling kit are sufficient to visualize DNA sequences from any tissue or organ. Despite the enormous progress of high-throughput sequencing technologies, DNA fiber-FISH remains a unique and indispensable tool to detect chromosomal rearrangements and to demonstrate differences between related species at high resolution. We discuss standard and alternative steps to easily prepare extended DNA fibers for high-resolution FISH mapping.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsT. Heitkam, S. Garcia
PublisherHumana Press
Pages351-363
Number of pages13
ISBN (Electronic)9781071632260
ISBN (Print)9781071632284
DOIs
Publication statusPublished - 20 Jun 2023

Publication series

NameMethods in Molecular Biology
Volume2672
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Chromatin fiber
  • Extended DNA fiber
  • FISH
  • Physical mapping

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