Expression pattern of ENOD40 in tomato roots upon infection with root-knot (Meloidogyne incognita) and cyst (Globodera rostochiensis) nematodes

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Early nodulin (ENOD) genes have been de¿ ned as genes expressed in legumes during nodule formation. Recent studies, however, revealed a number of homologues of ENOD40s in non-legume plants. Both in legumes and non-legumes ENOD40s are thought to play a role in the development of vascular tissues. In situ localisation of ENOD40 transcripts in root-knot nematode-infected roots of the legumeMedicagotruncatula revealed that this plant gene is expressed in and around giant cells at 6 days post infection (dpi). To test the expression of ENOD40 in a non-legume upon infection with root-knot and cyst nematodes, the expression of endogenous ENOD40 promoter-gusA fusion was studied in nematode-infected tomato roots at 1, 3, 7 and 14 dpi. In the main root, GUSstained areas were irregularly alternated with unstained areas. The lateral roots showed staining in the root-tip. Occasionally,GUS staining was observed in syncytia and giant cells. Sections from both GUS stained and nonstained feeding sites were investigated microscopically, and the results con¿ rmed our macroscopic observations. Hence, ENOD40 expression in tomato is basically unaffected by the induction of feeding sites by root-knot and cyst nematodes.
Original languageEnglish
Title of host publicationNematology Symposium abstracts
Place of PublicationLeiden
PublisherBrill Academic Publishers
Pages221 (281)
Volume4
Publication statusPublished - 2002

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