Expression of protocadherin gamma in skeletal muscle tissue is associated with age and muscle weakness

R.W.J. Hangelbroek, P. Fazelzadeh, C.A.B. Tieland, M.V. Boekschoten, G.J.E.J. Hooiveld, J.P.M. van Duynhoven, James Timmons, L. Verdijk, C.P.G.M. de Groot, L.J.C. van Loon, M.R. Müller

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Abstract

Background: The skeletal muscle system plays an important role in the independence of older adults. In this study we examine differences in the skeletal muscle transcriptome between healthy young and older subjects and (pre-)frail older adults. Additionally, we examine the effect of resistance-type exercise training on the muscle transcriptome in healthy older subjects and (pre-)frail older adults. Methods: Baseline transcriptome profiles were measured in muscle biopsies collected from 53 young, 73 healthy older subjects, and 61 frail older subjects. Follow-up samples from these frail older subjects (31 samples) and healthy older subjects (41 samples) were collected after 6 months of progressive resistance-type exercise training. Frail older subjects trained twice per week and the healthy older subjects trained three times per week. Results: At baseline genes related to mitochondrial function and energy metabolism were differentially expressed between older and young subjects, as well as between healthy and frail older subjects. Three hundred seven genes were differentially expressed after training in both groups. Training affected expression levels of genes related to extracellular matrix, glucose metabolism ,and vascularization. Expression of genes that were modulated by exercise training was indicative of muscle strength at baseline. Genes that strongly correlated with strength belonged to the protocadherin gamma gene cluster (r = −0.73). Conclusion: Our data suggest significant remaining plasticity of ageing skeletal muscle to adapt to resistance-type exercise training. Some age-related changes in skeletal muscle gene expression appear to be partially reversed by prolonged resistance-type exercise training. The protocadherin gamma gene cluster may be related to muscle denervation and re-innervation in ageing muscle.
Original languageEnglish
Pages (from-to)604-614
JournalJournal of cachexia, sarcopenia and muscle
Volume7
Issue number5
DOIs
Publication statusPublished - 2016

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Muscle Weakness
Resistance Training
Skeletal Muscle
Exercise
Healthy Volunteers
Muscles
Transcriptome
Frail Elderly
Multigene Family
Gene Expression
Muscle Denervation
Genes
Muscle Strength
Energy Metabolism
Extracellular Matrix
Gamma-protocadherins
Biopsy
Glucose

Cite this

@article{478b4923200a4521bbbabf96afa5abe0,
title = "Expression of protocadherin gamma in skeletal muscle tissue is associated with age and muscle weakness",
abstract = "Background: The skeletal muscle system plays an important role in the independence of older adults. In this study we examine differences in the skeletal muscle transcriptome between healthy young and older subjects and (pre-)frail older adults. Additionally, we examine the effect of resistance-type exercise training on the muscle transcriptome in healthy older subjects and (pre-)frail older adults. Methods: Baseline transcriptome profiles were measured in muscle biopsies collected from 53 young, 73 healthy older subjects, and 61 frail older subjects. Follow-up samples from these frail older subjects (31 samples) and healthy older subjects (41 samples) were collected after 6 months of progressive resistance-type exercise training. Frail older subjects trained twice per week and the healthy older subjects trained three times per week. Results: At baseline genes related to mitochondrial function and energy metabolism were differentially expressed between older and young subjects, as well as between healthy and frail older subjects. Three hundred seven genes were differentially expressed after training in both groups. Training affected expression levels of genes related to extracellular matrix, glucose metabolism ,and vascularization. Expression of genes that were modulated by exercise training was indicative of muscle strength at baseline. Genes that strongly correlated with strength belonged to the protocadherin gamma gene cluster (r = −0.73). Conclusion: Our data suggest significant remaining plasticity of ageing skeletal muscle to adapt to resistance-type exercise training. Some age-related changes in skeletal muscle gene expression appear to be partially reversed by prolonged resistance-type exercise training. The protocadherin gamma gene cluster may be related to muscle denervation and re-innervation in ageing muscle.",
author = "R.W.J. Hangelbroek and P. Fazelzadeh and C.A.B. Tieland and M.V. Boekschoten and G.J.E.J. Hooiveld and {van Duynhoven}, J.P.M. and James Timmons and L. Verdijk and {de Groot}, C.P.G.M. and {van Loon}, L.J.C. and M.R. M{\"u}ller",
year = "2016",
doi = "10.1002/jcsm.12099",
language = "English",
volume = "7",
pages = "604--614",
journal = "Journal of cachexia, sarcopenia and muscle",
issn = "2190-5991",
publisher = "Wiley",
number = "5",

}

Expression of protocadherin gamma in skeletal muscle tissue is associated with age and muscle weakness. / Hangelbroek, R.W.J.; Fazelzadeh, P.; Tieland, C.A.B.; Boekschoten, M.V.; Hooiveld, G.J.E.J.; van Duynhoven, J.P.M.; Timmons, James; Verdijk, L.; de Groot, C.P.G.M.; van Loon, L.J.C.; Müller, M.R.

In: Journal of cachexia, sarcopenia and muscle, Vol. 7, No. 5, 2016, p. 604-614.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Expression of protocadherin gamma in skeletal muscle tissue is associated with age and muscle weakness

AU - Hangelbroek, R.W.J.

AU - Fazelzadeh, P.

AU - Tieland, C.A.B.

AU - Boekschoten, M.V.

AU - Hooiveld, G.J.E.J.

AU - van Duynhoven, J.P.M.

AU - Timmons, James

AU - Verdijk, L.

AU - de Groot, C.P.G.M.

AU - van Loon, L.J.C.

AU - Müller, M.R.

PY - 2016

Y1 - 2016

N2 - Background: The skeletal muscle system plays an important role in the independence of older adults. In this study we examine differences in the skeletal muscle transcriptome between healthy young and older subjects and (pre-)frail older adults. Additionally, we examine the effect of resistance-type exercise training on the muscle transcriptome in healthy older subjects and (pre-)frail older adults. Methods: Baseline transcriptome profiles were measured in muscle biopsies collected from 53 young, 73 healthy older subjects, and 61 frail older subjects. Follow-up samples from these frail older subjects (31 samples) and healthy older subjects (41 samples) were collected after 6 months of progressive resistance-type exercise training. Frail older subjects trained twice per week and the healthy older subjects trained three times per week. Results: At baseline genes related to mitochondrial function and energy metabolism were differentially expressed between older and young subjects, as well as between healthy and frail older subjects. Three hundred seven genes were differentially expressed after training in both groups. Training affected expression levels of genes related to extracellular matrix, glucose metabolism ,and vascularization. Expression of genes that were modulated by exercise training was indicative of muscle strength at baseline. Genes that strongly correlated with strength belonged to the protocadherin gamma gene cluster (r = −0.73). Conclusion: Our data suggest significant remaining plasticity of ageing skeletal muscle to adapt to resistance-type exercise training. Some age-related changes in skeletal muscle gene expression appear to be partially reversed by prolonged resistance-type exercise training. The protocadherin gamma gene cluster may be related to muscle denervation and re-innervation in ageing muscle.

AB - Background: The skeletal muscle system plays an important role in the independence of older adults. In this study we examine differences in the skeletal muscle transcriptome between healthy young and older subjects and (pre-)frail older adults. Additionally, we examine the effect of resistance-type exercise training on the muscle transcriptome in healthy older subjects and (pre-)frail older adults. Methods: Baseline transcriptome profiles were measured in muscle biopsies collected from 53 young, 73 healthy older subjects, and 61 frail older subjects. Follow-up samples from these frail older subjects (31 samples) and healthy older subjects (41 samples) were collected after 6 months of progressive resistance-type exercise training. Frail older subjects trained twice per week and the healthy older subjects trained three times per week. Results: At baseline genes related to mitochondrial function and energy metabolism were differentially expressed between older and young subjects, as well as between healthy and frail older subjects. Three hundred seven genes were differentially expressed after training in both groups. Training affected expression levels of genes related to extracellular matrix, glucose metabolism ,and vascularization. Expression of genes that were modulated by exercise training was indicative of muscle strength at baseline. Genes that strongly correlated with strength belonged to the protocadherin gamma gene cluster (r = −0.73). Conclusion: Our data suggest significant remaining plasticity of ageing skeletal muscle to adapt to resistance-type exercise training. Some age-related changes in skeletal muscle gene expression appear to be partially reversed by prolonged resistance-type exercise training. The protocadherin gamma gene cluster may be related to muscle denervation and re-innervation in ageing muscle.

U2 - 10.1002/jcsm.12099

DO - 10.1002/jcsm.12099

M3 - Article

VL - 7

SP - 604

EP - 614

JO - Journal of cachexia, sarcopenia and muscle

JF - Journal of cachexia, sarcopenia and muscle

SN - 2190-5991

IS - 5

ER -