Expression of plant flavour genes in Lactococcus lactis

I. Hernández, D. Molenaar, M.J. Beekwilder, H.J. Bouwmeester, J.E.T. van Hylckama Vlieg

Research output: Contribution to journalArticleAcademicpeer-review

31 Citations (Scopus)

Abstract

Lactic acid bacteria, such as Lactococcus lactis, are attractive hosts for the production of plant-bioactive compounds because of their food grade status, efficient expression, and metabolic engineering tools. Two genes from strawberry (Fragaria x ananassa), encoding an alcohol acyltransferase (SAAT) and a linalool/nerolidol synthase (FaNES), were cloned in L. lactis and actively expressed using the nisin-induced expression system. The specific activity of SAAT could be improved threefold (up to 564 pmol octyl acetate h¿1 mg protein¿1) by increasing the concentration of tRNA1Arg, which is a rare tRNA molecule in L. lactis. Fermentation tests with GM17 medium and milk with recombinant L. lactis strains expressing SAAT or FaNES resulted in the production of octyl acetate (1.9 µM) and linalool (85 nM) to levels above their odor thresholds in water. The results illustrate the potential of the application of L. lactis as a food grade expression platform for the recombinant production of proteins and bioactive compounds from plants
Original languageEnglish
Pages (from-to)1544-1552
JournalApplied and Environmental Microbiology
Volume73
Issue number5
DOIs
Publication statusPublished - 2007

Fingerprint

Plant Genes
Lactococcus lactis
acetate
flavor
food
gene
Fragaria
food grades
milk
odor
fermentation
alcohol
linalool
genes
engineering
bacterium
protein
acetates
acid
Nisin

Keywords

  • alcohol acyl-transferase
  • escherichia-coli
  • acid bacteria
  • transfer-rna
  • heterologous expression
  • terpenoid metabolism
  • functional-analysis
  • molecular-cloning
  • dna microarrays
  • volatile esters

Cite this

Hernández, I., Molenaar, D., Beekwilder, M. J., Bouwmeester, H. J., & van Hylckama Vlieg, J. E. T. (2007). Expression of plant flavour genes in Lactococcus lactis. Applied and Environmental Microbiology, 73(5), 1544-1552. https://doi.org/10.1128/AEM.01870-06
Hernández, I. ; Molenaar, D. ; Beekwilder, M.J. ; Bouwmeester, H.J. ; van Hylckama Vlieg, J.E.T. / Expression of plant flavour genes in Lactococcus lactis. In: Applied and Environmental Microbiology. 2007 ; Vol. 73, No. 5. pp. 1544-1552.
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abstract = "Lactic acid bacteria, such as Lactococcus lactis, are attractive hosts for the production of plant-bioactive compounds because of their food grade status, efficient expression, and metabolic engineering tools. Two genes from strawberry (Fragaria x ananassa), encoding an alcohol acyltransferase (SAAT) and a linalool/nerolidol synthase (FaNES), were cloned in L. lactis and actively expressed using the nisin-induced expression system. The specific activity of SAAT could be improved threefold (up to 564 pmol octyl acetate h¿1 mg protein¿1) by increasing the concentration of tRNA1Arg, which is a rare tRNA molecule in L. lactis. Fermentation tests with GM17 medium and milk with recombinant L. lactis strains expressing SAAT or FaNES resulted in the production of octyl acetate (1.9 µM) and linalool (85 nM) to levels above their odor thresholds in water. The results illustrate the potential of the application of L. lactis as a food grade expression platform for the recombinant production of proteins and bioactive compounds from plants",
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Hernández, I, Molenaar, D, Beekwilder, MJ, Bouwmeester, HJ & van Hylckama Vlieg, JET 2007, 'Expression of plant flavour genes in Lactococcus lactis' Applied and Environmental Microbiology, vol. 73, no. 5, pp. 1544-1552. https://doi.org/10.1128/AEM.01870-06

Expression of plant flavour genes in Lactococcus lactis. / Hernández, I.; Molenaar, D.; Beekwilder, M.J.; Bouwmeester, H.J.; van Hylckama Vlieg, J.E.T.

In: Applied and Environmental Microbiology, Vol. 73, No. 5, 2007, p. 1544-1552.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Expression of plant flavour genes in Lactococcus lactis

AU - Hernández, I.

AU - Molenaar, D.

AU - Beekwilder, M.J.

AU - Bouwmeester, H.J.

AU - van Hylckama Vlieg, J.E.T.

PY - 2007

Y1 - 2007

N2 - Lactic acid bacteria, such as Lactococcus lactis, are attractive hosts for the production of plant-bioactive compounds because of their food grade status, efficient expression, and metabolic engineering tools. Two genes from strawberry (Fragaria x ananassa), encoding an alcohol acyltransferase (SAAT) and a linalool/nerolidol synthase (FaNES), were cloned in L. lactis and actively expressed using the nisin-induced expression system. The specific activity of SAAT could be improved threefold (up to 564 pmol octyl acetate h¿1 mg protein¿1) by increasing the concentration of tRNA1Arg, which is a rare tRNA molecule in L. lactis. Fermentation tests with GM17 medium and milk with recombinant L. lactis strains expressing SAAT or FaNES resulted in the production of octyl acetate (1.9 µM) and linalool (85 nM) to levels above their odor thresholds in water. The results illustrate the potential of the application of L. lactis as a food grade expression platform for the recombinant production of proteins and bioactive compounds from plants

AB - Lactic acid bacteria, such as Lactococcus lactis, are attractive hosts for the production of plant-bioactive compounds because of their food grade status, efficient expression, and metabolic engineering tools. Two genes from strawberry (Fragaria x ananassa), encoding an alcohol acyltransferase (SAAT) and a linalool/nerolidol synthase (FaNES), were cloned in L. lactis and actively expressed using the nisin-induced expression system. The specific activity of SAAT could be improved threefold (up to 564 pmol octyl acetate h¿1 mg protein¿1) by increasing the concentration of tRNA1Arg, which is a rare tRNA molecule in L. lactis. Fermentation tests with GM17 medium and milk with recombinant L. lactis strains expressing SAAT or FaNES resulted in the production of octyl acetate (1.9 µM) and linalool (85 nM) to levels above their odor thresholds in water. The results illustrate the potential of the application of L. lactis as a food grade expression platform for the recombinant production of proteins and bioactive compounds from plants

KW - alcohol acyl-transferase

KW - escherichia-coli

KW - acid bacteria

KW - transfer-rna

KW - heterologous expression

KW - terpenoid metabolism

KW - functional-analysis

KW - molecular-cloning

KW - dna microarrays

KW - volatile esters

U2 - 10.1128/AEM.01870-06

DO - 10.1128/AEM.01870-06

M3 - Article

VL - 73

SP - 1544

EP - 1552

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 5

ER -

Hernández I, Molenaar D, Beekwilder MJ, Bouwmeester HJ, van Hylckama Vlieg JET. Expression of plant flavour genes in Lactococcus lactis. Applied and Environmental Microbiology. 2007;73(5):1544-1552. https://doi.org/10.1128/AEM.01870-06