Expression of ENOD40 during tomato plant development

I.J.E. Vleghels; J.G.J. Hontelez; A. Ribeiro; P.F. Fransz; T. Bisseling; H.G.J.M. Franssen

doi:10.1007/s00425-003-1081-9

Expression of ENOD40 during tomato plant development

I.J.E. Vleghels, J.G.J. Hontelez, A. Ribeiro, P.F. Fransz, T. Bisseling, H.G.J.M. Franssen

Laboratory of Molecular Biology

Research output: Contribution to journal › Article › Academic › peer-review

16 Citations (Scopus)

Abstract

In legumes, ENOD40 expression is increased upon interaction of plants with rhizobia. Little is known of the expression pattern of ENOD40 during other stages of the plant life cycle. Studies of ENOD40 expression in non-legume development may give an indication of the function of the gene. To investigate the ENOD40 expression pattern during plant development, a fusion between the beta-glucuronidase (GUS) reporter gene and 150 bp of the 5' untranslated region plus 3,000 bp of 5' untranscribed tomato ENOD40 sequence was constructed and introduced into Lycopersicon esculentum Miller. Based on the observed GUS expression patterns in transgenic tomato we speculate that ENOD40 in tomato has a role in counteracting ethylene-provoked responses.

Original language	English
Pages (from-to)	42-49
Journal	Planta
Volume	218
DOIs	https://doi.org/10.1007/s00425-003-1081-9
Publication status	Published - 2003

Keywords

nodule development
medicago-truncatula
seed-germination
lotus-japonicus
binding protein
genes
patterns
mutant
pachytene
symbiosis

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title = "Expression of ENOD40 during tomato plant development",

abstract = "In legumes, ENOD40 expression is increased upon interaction of plants with rhizobia. Little is known of the expression pattern of ENOD40 during other stages of the plant life cycle. Studies of ENOD40 expression in non-legume development may give an indication of the function of the gene. To investigate the ENOD40 expression pattern during plant development, a fusion between the beta-glucuronidase (GUS) reporter gene and 150 bp of the 5' untranslated region plus 3,000 bp of 5' untranscribed tomato ENOD40 sequence was constructed and introduced into Lycopersicon esculentum Miller. Based on the observed GUS expression patterns in transgenic tomato we speculate that ENOD40 in tomato has a role in counteracting ethylene-provoked responses.",

keywords = "nodule development, medicago-truncatula, seed-germination, lotus-japonicus, binding protein, genes, patterns, mutant, pachytene, symbiosis",

author = "I.J.E. Vleghels and J.G.J. Hontelez and A. Ribeiro and P.F. Fransz and T. Bisseling and H.G.J.M. Franssen",

year = "2003",

doi = "10.1007/s00425-003-1081-9",

language = "English",

volume = "218",

pages = "42--49",

journal = "Planta",

issn = "0032-0935",

publisher = "Springer",

}

TY - JOUR

T1 - Expression of ENOD40 during tomato plant development

AU - Vleghels, I.J.E.

AU - Hontelez, J.G.J.

AU - Ribeiro, A.

AU - Fransz, P.F.

AU - Bisseling, T.

AU - Franssen, H.G.J.M.

PY - 2003

Y1 - 2003

N2 - In legumes, ENOD40 expression is increased upon interaction of plants with rhizobia. Little is known of the expression pattern of ENOD40 during other stages of the plant life cycle. Studies of ENOD40 expression in non-legume development may give an indication of the function of the gene. To investigate the ENOD40 expression pattern during plant development, a fusion between the beta-glucuronidase (GUS) reporter gene and 150 bp of the 5' untranslated region plus 3,000 bp of 5' untranscribed tomato ENOD40 sequence was constructed and introduced into Lycopersicon esculentum Miller. Based on the observed GUS expression patterns in transgenic tomato we speculate that ENOD40 in tomato has a role in counteracting ethylene-provoked responses.

AB - In legumes, ENOD40 expression is increased upon interaction of plants with rhizobia. Little is known of the expression pattern of ENOD40 during other stages of the plant life cycle. Studies of ENOD40 expression in non-legume development may give an indication of the function of the gene. To investigate the ENOD40 expression pattern during plant development, a fusion between the beta-glucuronidase (GUS) reporter gene and 150 bp of the 5' untranslated region plus 3,000 bp of 5' untranscribed tomato ENOD40 sequence was constructed and introduced into Lycopersicon esculentum Miller. Based on the observed GUS expression patterns in transgenic tomato we speculate that ENOD40 in tomato has a role in counteracting ethylene-provoked responses.

KW - nodule development

KW - medicago-truncatula

KW - seed-germination

KW - lotus-japonicus

KW - binding protein

KW - genes

KW - patterns

KW - mutant

KW - pachytene

KW - symbiosis

U2 - 10.1007/s00425-003-1081-9

DO - 10.1007/s00425-003-1081-9

M3 - Article

SN - 0032-0935

VL - 218

SP - 42

EP - 49

JO - Planta

JF - Planta

ER -

Expression of ENOD40 during tomato plant development

Abstract

Keywords

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