Expression of defender against apoptotic death (DAD-1) in iris and dianthus petals

D.A.M. van der Kop, G. Ruys, D. Dees, C. van der Schoot, A.D. de Boer, W.G. van Doorn

    Research output: Contribution to journalArticleAcademicpeer-review

    36 Citations (Scopus)

    Abstract

    The gene defender against apoptotic death (DAD-1) prevents programmed cell death in animal cells. We investigated the expression pattern of DAD-1 in petals of iris (Iris x hollandica cv. Blue Magic) and carnation (Dianthus caryophyllus cv. Etarro). DAD-1 expression in Iris petals was strongly reduced by the time of visible senescence, which occurs 4 days after flower opening. Microscopic analysis showed that most mesophyll cells had died prior to a clear decrease in DAD-1 expression and that epidermis cells started to die by that time. In carnation petals DAD-1 expression also decreased by the time of massive cell death. After ethylene treatment, DAD-1 expression in carnation again decreased concomitant with the advance in massive cell death. In conclusion, DAD-1 is not an early regulator of petal cell death. Its expression may be required for the programmed dismantling of cells, as it ceases only just prior to, or concomitant with, cell death.
    Original languageEnglish
    Pages (from-to)256-263
    JournalPhysiologia Plantarum
    Volume117
    Issue number2
    DOIs
    Publication statusPublished - 2003

    Keywords

    • programmed cell-death
    • molecular-cloning
    • mammalian oligosaccharyltransferase
    • flower petals
    • senescence
    • protein
    • homolog
    • suppressor
    • cdna
    • gene

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