TY - JOUR
T1 - Expression of Clarkia S-linalool synthase in transgenic petunia plant results in the accumulation of S-linalyl-b-D-glucopyranoside
AU - Lücker, J.
AU - Bouwmeester, H.J.
AU - Schwab, W.
AU - Blaas, J.
AU - van der Plas, L.H.W.
AU - Verhoeven, H.A.
PY - 2001
Y1 - 2001
N2 - Petunia hybrida W115 was transformed with a Clarkia breweri S-linalool synthase cDNA (lis). Lis was expressed in all tissues analysed, and linalool was detected in leaves, sepals, corolla, stem and ovary, but not in nectaries, roots, pollen and style. However, the S-linalool produced by the plant in the various tissues is not present as free linalool, but was efficiently converted to non-volatile S-linalyl--d-glucopyranoside by the action of endogenous glucosyltransferase. The results presented demonstrate that monoterpene production can be altered by genetic modification, and that the compounds produced can be converted by endogenous enzymatic activity.
AB - Petunia hybrida W115 was transformed with a Clarkia breweri S-linalool synthase cDNA (lis). Lis was expressed in all tissues analysed, and linalool was detected in leaves, sepals, corolla, stem and ovary, but not in nectaries, roots, pollen and style. However, the S-linalool produced by the plant in the various tissues is not present as free linalool, but was efficiently converted to non-volatile S-linalyl--d-glucopyranoside by the action of endogenous glucosyltransferase. The results presented demonstrate that monoterpene production can be altered by genetic modification, and that the compounds produced can be converted by endogenous enzymatic activity.
KW - Liquid chromatography-tandem MS
KW - Monoterpene-glycoside
KW - Monoterpenoids
KW - Pathway engineering
KW - Petunia hybrida
KW - S-linalool synthase
U2 - 10.1046/j.1365-313x.2001.01097.x
DO - 10.1046/j.1365-313x.2001.01097.x
M3 - Article
SN - 0960-7412
VL - 27
SP - 315
EP - 324
JO - The Plant Journal
JF - The Plant Journal
ER -