Expression of beta-defensins pBD-1 and pBD-2 along the small tract of the pig: Lack of upregulation in vivo upon Salmonella typhimurium infection

E.J. Veldhuizen, A. van Dijk, M.H. Tersteeg, S.I. Kalkhove, J. van der Meulen, T.A. Niewold, H.P. Haagsman

Research output: Contribution to journalArticleAcademicpeer-review

50 Citations (Scopus)

Abstract

Defensins are antimicrobial peptides that play an important role in the innate immune response in the intestine. Up to date, only one ß-defensin (pBD-1), has been described in pig, which was found to be expressed at low levels in the intestine. We set-up a quantitative PCR method to detect the gene expression of pBD-1 and a newly discovered porcine ß-defensin, pBD-2. Expression of pBD-1 mRNA increased from the proximal to the distal part of the intestine whereas pBD-2 expression decreased. The main gene expression sites for pBD-2 were kidney and liver, whereas pBD-1 was mainly expressed in tongue. The porcine small intestinal segment perfusion (SISP) technique was used to investigate effects of Salmonella typhimurium DT104 on intestinal morphology and pBD-1 and pBD-2 mRNA levels in vivo. The early responses were studied 2, 4 and 8 h post-infection in four separate jejunal and ileal segments. Immunohistochemistry showed invasion of the mucosa by Salmonella and changes in intestinal morphology. However, no concomitant changes in expression of either pBD-1 or pBD-2 were observed. We conclude that at least two defensins are differentially expressed in the intestine of pigs, and that expression of both defensins is not altered by S. typhimurium under these conditions.
Original languageEnglish
Pages (from-to)276-283
JournalMolecular Immunology
Volume44
Issue number4
DOIs
Publication statusPublished - 2007

Keywords

  • human beta-defensin-2
  • molecular-cloning
  • innate immunity
  • mast-cells
  • peptide
  • gene
  • disease
  • pcr
  • quantitation
  • tissue

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