Expression of alternansucrase in potato plants

G.A. Kok-Jacon, J.P. Vincken, Denong Wang, Shaoyi Liu, R.G.F. Visser

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Alternan, which consists of alternating ¿-(1¿3)/¿-(1¿6)-linked glucosyl residues, was produced in potato tubers by expressing a mature alternansucrase (Asr) gene from Leuconostoc mesenteroides NRRL B-1355 in potato. Detection of alternan was performed by enzyme-linked immunosorbent assay in tuber juices, revealing a concentration between 0.3 and 1.2 mg g-1 fresh wt. The Asr transcript levels correlated well with alternan accumulation in tuber juices. It appeared that the expression of sucrose-regulated starch-synthesizing genes (ADP-glucose pyrophosphorylase subunit S and granule-bound starch synthase I) was down-regulated. Despite this, the physico-chemical properties of the transgenic starches were unaltered. These results are compared to those obtained with other transgenic potato plants producing mutan [¿-(1¿3)-linked glucosyl residues] and dextran [¿-(1¿6)-linked glucosyl residues].
Original languageEnglish
Pages (from-to)1135-1142
JournalBiotechnology Letters
Volume29
Issue number7
DOIs
Publication statusPublished - 2007

Keywords

  • leuconostoc-mesenteroides
  • transgenic plants
  • sucrose
  • gene
  • starch
  • biosynthesis
  • dextran

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