Expression of a ribosomal protein gene during germination of cabbage (Brassica oleracea f. oleracea) seeds

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

Abstract

A study was conducted to investigate the hypothesis that the expression of ribosomal protein genes is correlated with germination performance. Cabbage cv. Bartolo seeds were germinated under 25 or 35°C in darkness or in red light (652 nm, 26 W/m2), and imbibed either in water or in polyethylene glycol (PEG). The expression of the AtRPL27B and AtRPL36B genes were studied in relation to germination performance, using Arabidopsis thaliana genes as heterologous probes in Northern analysis. Both genes showed marginal expression in dry seeds, and increased expression was observed during germination in water. The expression decreased with increasing osmotic stress. The expression of both genes after 18 h in either water or PEG correlated with maximum germination and inversely with the period to reach 50% germination. The cabbage clone BoRPL27 showed high nucleotide similarity to putative A. thaliana RPL27 homologues located at chromosome 3.
Original languageEnglish
Title of host publicationThe Biology of Seeds: Recent Research Advances: proceedings of the seventh international workshop on seeds, Salamanca, Spain 2002
EditorsG. Nicolas, K.J. Bradford, D. Come, H.W. Pritchard
Place of PublicationWallingford
PublisherCAB International
Chapter21
Pages191-197
Number of pages7
ISBN (Print)9780851996530
DOIs
Publication statusPublished - 2003

Keywords

  • brassica oleracea
  • seed germination
  • gene expression
  • ribosomes
  • proteins
  • ribosomal rna

Fingerprint Dive into the research topics of 'Expression of a ribosomal protein gene during germination of cabbage (Brassica oleracea f. oleracea) seeds'. Together they form a unique fingerprint.

Cite this