Expression analyses of salinity stress- associated ESTs in Aeluropus littoralis

Tayebeh Kakeshpour, Sajad Rashidi Monfared, Amin Ebrahimi*, Arman Beyraghdar Kashkooli, Esmaeil Ebrahimie

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Salinity is among the most important abiotic stresses affecting crop production throughout the earth. Halophyte plants can sustain high salinity levels, therefore elucidating molecular mechanisms underlying their salinity resistance is beneficial for crop improvement. Aeluropus littoralis, a halophyte weed, is a great genetic resource for this purpose. Isolated expressed sequence taq (EST) sequences from A. littoralis under salinity stress, have given us the chance to find and analyze transcripts of genes involved in response to salinity. Transcriptome analyses indicated the expression levels of mRNAs corresponding to 10 of sequences were increased under treatments. All mRNAs were significantly induced under salt treatment with the highest peaks observed at different hours of treatments. Moreover, the full-length cDNA of vacuolar H+-pyrophosphatase (VP) was isolated utilizing 3′ and 5′ rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR) and characterized (GenBank accession number of KT253223.1). The extracted full-length of VP was 2732 bp, which contained ORF of 2292 bp encoding 763 amino acids.

Original languageEnglish
Pages (from-to)76-84
JournalGene Expression Patterns
Volume25-26
DOIs
Publication statusPublished - 1 Nov 2017

Keywords

  • 3′ and 5′ RACE-PCR
  • Aeluropus littoralis
  • EST sequences
  • qRT-PCR
  • Salt stress

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