Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.).

B. Studer, T. Asp, U. Frei, S. Hentrup, H. Meally, A. Guillard, S. Barth, H. Muylle, I. Roldan-Ruiz, P. Barre, C.F.S. Boucoiran, G. Stunnenberg, O. Dolstra, L. Skot, K.P. Skot, B. Turner, M. Humphreys, R. Kolliker, N. Roulund, K.K. NielsenT. Lubberstedt

    Research output: Contribution to journalArticleAcademicpeer-review

    26 Citations (Scopus)

    Abstract

    An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses. Electronic supplementary material The online version of this article (doi:10.1007/s11032-007-9148-0) contains supplementary material, which is available to authorized users.
    Original languageEnglish
    Pages (from-to)533-548
    JournalMolecular Breeding
    Volume21
    Issue number4
    DOIs
    Publication statusPublished - 2008

    Keywords

    • repeat ssr markers
    • multiflorum lam.
    • linkage map
    • plants
    • transferability
    • construction
    • reveals
    • qtl

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