Persistent nematode infections are a major threat to important food crops. These round worms manipulate plant cell morphology and physiology to establish sophisticated feeding structures. Modifications to plant cells are largely attributed to the activity of nematode secreted effectors. SPRYSECs are a remarkably expanded family of effectors identified initially in the potato cyst nematode Globodera rostochiensis. In the sibling species Globodera pallida, a SPRYSEC subfamily is present called RBP-1. Although some members are specifically recognized by the Gpa2 resistance gene from potato, their role in nematode virulence is still unknown. To address this question, we performed a. Y2H screening of a nematode-infected susceptible potato library to identify host targets involved in nematode parasitism. This yielded a number of interacting candidates involved in post-translational modification in plants. We have independently confirmed that two ligases involved in post-translational modification can interact with both virulent and avirulent variants of Rbp-1 in yeast. A localization study also shows that the candidate interactors localize to the nucleus, which allows interaction with Rpb-1 as it shows a nucleocytoplasmic localization pattern. Upon co-expression of the interactors, a shift towards to nucleus was observed for RBP-1 suggesting that they reside indeed in the same complex. Furthermore, upon silencing of the corresponding ligase genes in A. thaliana, we observed significant differences in the amount of nematodes present in the roots of nematode infected plants, indicating their importance for nematode parasitism. These candidate interactors of Rbp-1 suggest that the intrinsic role of the effector is carried out through manipulation of the plant post-translational modification machinery. Our findings suggest that nematodes are able to use this repertoire of effectors to control different aspects of the plant cell to establish a feeding site. Therefore our results may provide further insight into the basis of virulence of nematodes in plants.
|Number of pages||1|
|Publication status||Published - 17 Feb 2016|
|Event||3rd Annual Conference of the COST Action Sustain (FA1208) - Banyuls s/ Mer, France|
Duration: 17 Feb 2016 → 19 Feb 2016
|Conference||3rd Annual Conference of the COST Action Sustain (FA1208)|
|City||Banyuls s/ Mer|
|Period||17/02/16 → 19/02/16|
Diaz Granados Muñoz, A., Overmars, H. A., Ariaans, R., van Schaik, C. C., Slootweg, E. J., Bakker, J., Smant, G., & Goverse, A. (2016). Exploring the role of Rbp-1 in Globodera pallida parasitism. 53. Poster session presented at 3rd Annual Conference of the COST Action Sustain (FA1208), Banyuls s/ Mer, France.