Exploring the involvement of Alternative respiratory pathway in Pisum sativum L. seed germination

L. Rodrigues*, R. Claudino, S.P.C. Groot, P. Hohmann, A. Nogales, L. Hansen, H. Cardoso*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademicpeer-review


Organic agriculture, recognized as a more sustainable agricultural system, strongly de-pends on the use of highly resilient genotypes. Resilient seeds, with increased tolerance to germinate and provide vigorous seedlings under environmental stresses, currently represent one of the most important agronomical traits. Seed germination involves the activation of several metabolic path-ways, including cellular respiration. Alternative oxidase (AOX), a key enzyme in the alternative respiratory pathway, plays a crucial role in regulating cell reprogramming by controlling metabolic transitions related to the cellular redox state and the variable carbon balance. The involvement of the alternative respiratory pathway during germination was explored by analysis of PsAOX gene/protein expression. Seeds of four Pisum sativum L. cultivars (‘Respect-1′, ‘S134′, ‘G78′ and ‘S91′) were imbibed in sterile tap water for 16 h and metabolic parameters measured by calorespirometry (heat and CO2 emission rates) in a Multi-Cell Differential Scanning Calorimeter in isothermal mode at 25 °C. The involvement of PsAOX was evaluated by transcript quantification (PsAOX1, PsAOX2a, and PsAOX2b) through RT-qPCR, and by of analysing the PsAOX expression through Western blot. The results demonstrate that the cv. ‘S91′, characterized by a low germination rate, exhibited the lowest metabolic heat and CO2 emission rate. However, contrary to expectations, PsAOX transcript accumulation and PsAOX protein expression were significantly higher for ‘S91′ than for the other cultivars. These results indicate that higher levels of AOX (transcript and protein) could be linked to lower metabolic rates for embryo growth when seed germination is compromised.
Original languageEnglish
Title of host publicationProceedings of The 1st International Electronic Conference on Horticulturae
EditorsJ.A. Fernández, J.A. Pascual, Y. Zhang
Number of pages6
Publication statusPublished - 15 Apr 2022
Event1st International Electronic Conference on Horticulturae (2022) - Basel, Switzerland
Duration: 16 Apr 202230 Apr 2022

Publication series

NameBiology and Life Sciences Forum
ISSN (Electronic)2673-9976


Conference1st International Electronic Conference on Horticulturae (2022)


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