Exploration of microbial systems as biocatalysts for conversion of synthesis gas to bio-based chemicals

Research output: Thesisinternal PhD, WU

Abstract

Synthesis gas (syngas) fermentation is a process capable of processing a gaseous substrate via fermentation into commodity chemicals and fuels. Gas (mainly consisting of hydrogen, carbon monoxide and carbon dioxide) fed to the fermentation process can be obtained from a wide variety of sources, including off-gases from industry, gasification of solid carbon wastes (e.g. municipal waste, lignocellulosic biomass) or gas derived from electrochemical reduction/physicochemical reduction processes.

Current limitations of the fermentation process are the relatively poorly understood physiology and genetics of the biocatalysts involved. Therefore the work described in this thesis aimed at unravelling of the syngas metabolism of acetogenic and methanogenic strains, with main focus on carbon monoxide metabolism. In addition, the application of synthetic co-cultures for syngas fermentation was explored in order to assess if such cultivation approach could lead to broadening of the syngas fermentation product spectrum. In addition to co-cultivation proof-of-concept studies for application, new fundamental insights on the metabolism of the involved biocatalysts were obtained.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Stams, Fons, Promotor
  • Machado de Sousa, Diana, Co-promotor
Award date8 Feb 2019
Place of PublicationWageningen
Publisher
Print ISBNs9789463433914
DOIs
Publication statusPublished - 2019

Fingerprint

Synthesis gas
Fermentation
Enzymes
Metabolism
Gases
Carbon Monoxide
Physiology
Gasification
Carbon Dioxide
Hydrogen
Biomass
Carbon
Substrates
Processing
Industry

Keywords

  • cum laude

Cite this

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title = "Exploration of microbial systems as biocatalysts for conversion of synthesis gas to bio-based chemicals",
abstract = "Synthesis gas (syngas) fermentation is a process capable of processing a gaseous substrate via fermentation into commodity chemicals and fuels. Gas (mainly consisting of hydrogen, carbon monoxide and carbon dioxide) fed to the fermentation process can be obtained from a wide variety of sources, including off-gases from industry, gasification of solid carbon wastes (e.g. municipal waste, lignocellulosic biomass) or gas derived from electrochemical reduction/physicochemical reduction processes. Current limitations of the fermentation process are the relatively poorly understood physiology and genetics of the biocatalysts involved. Therefore the work described in this thesis aimed at unravelling of the syngas metabolism of acetogenic and methanogenic strains, with main focus on carbon monoxide metabolism. In addition, the application of synthetic co-cultures for syngas fermentation was explored in order to assess if such cultivation approach could lead to broadening of the syngas fermentation product spectrum. In addition to co-cultivation proof-of-concept studies for application, new fundamental insights on the metabolism of the involved biocatalysts were obtained.",
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author = "Martijn Diender",
note = "WU thesis 7151 Titel on cover: Exploration of microbial systems as biocatalysts for conversion of syngas to bio-based chemicals Includes bibliographical references. - With summaries in English and Dutch",
year = "2019",
doi = "10.18174/466065",
language = "English",
isbn = "9789463433914",
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Exploration of microbial systems as biocatalysts for conversion of synthesis gas to bio-based chemicals. / Diender, Martijn.

Wageningen : Wageningen University, 2019. 204 p.

Research output: Thesisinternal PhD, WU

TY - THES

T1 - Exploration of microbial systems as biocatalysts for conversion of synthesis gas to bio-based chemicals

AU - Diender, Martijn

N1 - WU thesis 7151 Titel on cover: Exploration of microbial systems as biocatalysts for conversion of syngas to bio-based chemicals Includes bibliographical references. - With summaries in English and Dutch

PY - 2019

Y1 - 2019

N2 - Synthesis gas (syngas) fermentation is a process capable of processing a gaseous substrate via fermentation into commodity chemicals and fuels. Gas (mainly consisting of hydrogen, carbon monoxide and carbon dioxide) fed to the fermentation process can be obtained from a wide variety of sources, including off-gases from industry, gasification of solid carbon wastes (e.g. municipal waste, lignocellulosic biomass) or gas derived from electrochemical reduction/physicochemical reduction processes. Current limitations of the fermentation process are the relatively poorly understood physiology and genetics of the biocatalysts involved. Therefore the work described in this thesis aimed at unravelling of the syngas metabolism of acetogenic and methanogenic strains, with main focus on carbon monoxide metabolism. In addition, the application of synthetic co-cultures for syngas fermentation was explored in order to assess if such cultivation approach could lead to broadening of the syngas fermentation product spectrum. In addition to co-cultivation proof-of-concept studies for application, new fundamental insights on the metabolism of the involved biocatalysts were obtained.

AB - Synthesis gas (syngas) fermentation is a process capable of processing a gaseous substrate via fermentation into commodity chemicals and fuels. Gas (mainly consisting of hydrogen, carbon monoxide and carbon dioxide) fed to the fermentation process can be obtained from a wide variety of sources, including off-gases from industry, gasification of solid carbon wastes (e.g. municipal waste, lignocellulosic biomass) or gas derived from electrochemical reduction/physicochemical reduction processes. Current limitations of the fermentation process are the relatively poorly understood physiology and genetics of the biocatalysts involved. Therefore the work described in this thesis aimed at unravelling of the syngas metabolism of acetogenic and methanogenic strains, with main focus on carbon monoxide metabolism. In addition, the application of synthetic co-cultures for syngas fermentation was explored in order to assess if such cultivation approach could lead to broadening of the syngas fermentation product spectrum. In addition to co-cultivation proof-of-concept studies for application, new fundamental insights on the metabolism of the involved biocatalysts were obtained.

KW - cum laude

U2 - 10.18174/466065

DO - 10.18174/466065

M3 - internal PhD, WU

SN - 9789463433914

PB - Wageningen University

CY - Wageningen

ER -