Experimental nasal colonization of piglets with methicillin-susceptible and methicillin-resistant Staphylococcus aureus

Koen M. Verstappen, Birgitta Duim, Arie van Nes, Susan Snijders, Willem J.B. van Wamel, Jaap A. Wagenaar*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Methicillin-resistant Staphylococcus aureus sequence type (ST)398 is widely spread among livestock. People in contact with livestock have a higher risk of testing positive for MRSA. Several experimental settings have been described to study in vivo colonization of MRSA in pigs, each having its own limitations. The aim of this study was to develop a nose-colonization model in pigs to quantitatively study the colonization of MRSA and the co-colonization of MSSA and MRSA.Two experiments were performed: in the first experiment piglets received an intranasal inoculation with MRSA ST398, spa-type t011, and in the second experiment piglets received an intranasal inoculation with two MSSA strains (ST398, spa-type t011 and t034) and two MRSA strains (also ST398, spa-type t011 and t034) to investigate co-colonization. Colonization was quantitatively monitored for 2 weeks in both experiments.Nasal colonization was successfully established in all piglets with stable numbers of S. aureus between 104 and 106CFU. MSSA and MRSA were able to co-colonize.

Original languageEnglish
Pages (from-to)483-488
Number of pages6
JournalVeterinary Microbiology
Volume174
Issue number3-4
DOIs
Publication statusPublished - Dec 2014

Keywords

  • Co-colonization
  • MRSA
  • Nasal colonization
  • Pigs
  • S. aureus

Fingerprint

Dive into the research topics of 'Experimental nasal colonization of piglets with methicillin-susceptible and methicillin-resistant Staphylococcus aureus'. Together they form a unique fingerprint.

Cite this