Experimental infection of small ruminants with bluetongue virus expressing Toggenburg Orbivirus proteins

Piet A. van Rijn, Sandra G.P. van de Water, Mieke A. Maris-Veldhuis, René G.P. van Gennip

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Bluetongue virus (BTV) is the prototype orbivirus (Reoviridae family, genus Orbivirus) consisting of more than 24 recognized serotypes or neutralization groups. Recently, new BTV serotypes in goats have been found; serotype 25 (Toggenburg Orbivirusor TOV), serotype 26 (KUW2010/02), and serotype 27 from Corsica, France. KUW2010/02 has been isolated in mammalian cells but is not replicating in Culicoides cells. TOVhas been detected in goats but could not been cultured, although TOV has been successfully passed to naïve animals by experimental infection using viremic blood. Genome segments Seg-2[VP2], Seg-6[VP5], Seg-7[VP7], and Seg-10[NS3/NS3a] expressing the respective TOV proteins were incorporated in BTV using reverse genetics, demonstrating that these TOV proteins are functional in BTV replication. Depending on the incorporated TOV proteins, in vitro replication is, however, decreased compared to the ancestor BTV, in particular by TOV-VP5. Sheep and goats were experimentally infected with BTV expressing both outer capsid proteins VP2 and VP5 of TOV, so-named ‘TOV-serotyped BTV’. Viremia was not detected in sheep, and hardly detected in goats after infection with TOV-serotyped BTV. Seroconversion by cELISA, however, was detected, suggesting that TOV-serotyped BTV replicates in small ruminants. One goat was coincidentally pregnant, and the fetus was strong PCR-positive in blood samples and several organs, which conclusively demonstrates that TOV-serotyped BTV replicates in vivo.

Original languageEnglish
Pages (from-to)145-151
JournalVeterinary Microbiology
Volume192
DOIs
Publication statusPublished - 2016

Fingerprint

Orbivirus
Toggenburg
Bluetongue virus
Ruminants
small ruminants
Infection
Goats
infection
serotypes
Proteins
proteins
goats
France
Sheep
Reoviridae
Ceratopogonidae
Reverse Genetics
sheep
Culicoides
Corsica

Keywords

  • Bluetongue virus
  • Experimental infection
  • Goat
  • Protein expression
  • Reverse genetics
  • Sheep
  • Toggenburg Orbivirus

Cite this

van Rijn, Piet A. ; van de Water, Sandra G.P. ; Maris-Veldhuis, Mieke A. ; van Gennip, René G.P. / Experimental infection of small ruminants with bluetongue virus expressing Toggenburg Orbivirus proteins. In: Veterinary Microbiology. 2016 ; Vol. 192. pp. 145-151.
@article{fcd86405ce6d4f18a368b44af19ecf30,
title = "Experimental infection of small ruminants with bluetongue virus expressing Toggenburg Orbivirus proteins",
abstract = "Bluetongue virus (BTV) is the prototype orbivirus (Reoviridae family, genus Orbivirus) consisting of more than 24 recognized serotypes or neutralization groups. Recently, new BTV serotypes in goats have been found; serotype 25 (Toggenburg Orbivirusor TOV), serotype 26 (KUW2010/02), and serotype 27 from Corsica, France. KUW2010/02 has been isolated in mammalian cells but is not replicating in Culicoides cells. TOVhas been detected in goats but could not been cultured, although TOV has been successfully passed to na{\"i}ve animals by experimental infection using viremic blood. Genome segments Seg-2[VP2], Seg-6[VP5], Seg-7[VP7], and Seg-10[NS3/NS3a] expressing the respective TOV proteins were incorporated in BTV using reverse genetics, demonstrating that these TOV proteins are functional in BTV replication. Depending on the incorporated TOV proteins, in vitro replication is, however, decreased compared to the ancestor BTV, in particular by TOV-VP5. Sheep and goats were experimentally infected with BTV expressing both outer capsid proteins VP2 and VP5 of TOV, so-named ‘TOV-serotyped BTV’. Viremia was not detected in sheep, and hardly detected in goats after infection with TOV-serotyped BTV. Seroconversion by cELISA, however, was detected, suggesting that TOV-serotyped BTV replicates in small ruminants. One goat was coincidentally pregnant, and the fetus was strong PCR-positive in blood samples and several organs, which conclusively demonstrates that TOV-serotyped BTV replicates in vivo.",
keywords = "Bluetongue virus, Experimental infection, Goat, Protein expression, Reverse genetics, Sheep, Toggenburg Orbivirus",
author = "{van Rijn}, {Piet A.} and {van de Water}, {Sandra G.P.} and Maris-Veldhuis, {Mieke A.} and {van Gennip}, {Ren{\'e} G.P.}",
year = "2016",
doi = "10.1016/j.vetmic.2016.07.013",
language = "English",
volume = "192",
pages = "145--151",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",

}

Experimental infection of small ruminants with bluetongue virus expressing Toggenburg Orbivirus proteins. / van Rijn, Piet A.; van de Water, Sandra G.P.; Maris-Veldhuis, Mieke A.; van Gennip, René G.P.

In: Veterinary Microbiology, Vol. 192, 2016, p. 145-151.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Experimental infection of small ruminants with bluetongue virus expressing Toggenburg Orbivirus proteins

AU - van Rijn, Piet A.

AU - van de Water, Sandra G.P.

AU - Maris-Veldhuis, Mieke A.

AU - van Gennip, René G.P.

PY - 2016

Y1 - 2016

N2 - Bluetongue virus (BTV) is the prototype orbivirus (Reoviridae family, genus Orbivirus) consisting of more than 24 recognized serotypes or neutralization groups. Recently, new BTV serotypes in goats have been found; serotype 25 (Toggenburg Orbivirusor TOV), serotype 26 (KUW2010/02), and serotype 27 from Corsica, France. KUW2010/02 has been isolated in mammalian cells but is not replicating in Culicoides cells. TOVhas been detected in goats but could not been cultured, although TOV has been successfully passed to naïve animals by experimental infection using viremic blood. Genome segments Seg-2[VP2], Seg-6[VP5], Seg-7[VP7], and Seg-10[NS3/NS3a] expressing the respective TOV proteins were incorporated in BTV using reverse genetics, demonstrating that these TOV proteins are functional in BTV replication. Depending on the incorporated TOV proteins, in vitro replication is, however, decreased compared to the ancestor BTV, in particular by TOV-VP5. Sheep and goats were experimentally infected with BTV expressing both outer capsid proteins VP2 and VP5 of TOV, so-named ‘TOV-serotyped BTV’. Viremia was not detected in sheep, and hardly detected in goats after infection with TOV-serotyped BTV. Seroconversion by cELISA, however, was detected, suggesting that TOV-serotyped BTV replicates in small ruminants. One goat was coincidentally pregnant, and the fetus was strong PCR-positive in blood samples and several organs, which conclusively demonstrates that TOV-serotyped BTV replicates in vivo.

AB - Bluetongue virus (BTV) is the prototype orbivirus (Reoviridae family, genus Orbivirus) consisting of more than 24 recognized serotypes or neutralization groups. Recently, new BTV serotypes in goats have been found; serotype 25 (Toggenburg Orbivirusor TOV), serotype 26 (KUW2010/02), and serotype 27 from Corsica, France. KUW2010/02 has been isolated in mammalian cells but is not replicating in Culicoides cells. TOVhas been detected in goats but could not been cultured, although TOV has been successfully passed to naïve animals by experimental infection using viremic blood. Genome segments Seg-2[VP2], Seg-6[VP5], Seg-7[VP7], and Seg-10[NS3/NS3a] expressing the respective TOV proteins were incorporated in BTV using reverse genetics, demonstrating that these TOV proteins are functional in BTV replication. Depending on the incorporated TOV proteins, in vitro replication is, however, decreased compared to the ancestor BTV, in particular by TOV-VP5. Sheep and goats were experimentally infected with BTV expressing both outer capsid proteins VP2 and VP5 of TOV, so-named ‘TOV-serotyped BTV’. Viremia was not detected in sheep, and hardly detected in goats after infection with TOV-serotyped BTV. Seroconversion by cELISA, however, was detected, suggesting that TOV-serotyped BTV replicates in small ruminants. One goat was coincidentally pregnant, and the fetus was strong PCR-positive in blood samples and several organs, which conclusively demonstrates that TOV-serotyped BTV replicates in vivo.

KW - Bluetongue virus

KW - Experimental infection

KW - Goat

KW - Protein expression

KW - Reverse genetics

KW - Sheep

KW - Toggenburg Orbivirus

U2 - 10.1016/j.vetmic.2016.07.013

DO - 10.1016/j.vetmic.2016.07.013

M3 - Article

VL - 192

SP - 145

EP - 151

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

ER -