Evidence that the essential response regulator YycF in Streptococcus pneumoniae modulates expression of fatty acid biosynthesis genes and alters membrane composition

Luz Mohedano, Karin Overweg, Alicia De La Fuente, Mark Reuter, Silvia Altabe, Francis Mulholland, Diego De Mendoza, Paloma López*, Jerry M. Wells

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

87 Citations (Scopus)

Abstract

The YycFG two-component system, originally identified in Bacillus subtilis, is highly conserved among gram-positive bacteria with low G+C contents. In Streptococcus pneumoniae, the YycF response regulator has been reported to be essential for cell growth, but the signal to which it responds and the gene members of the regulon remain unclear. In order to investigate the role of YycFG in S. pneumoniae, we increased the expression of yycF by using a maltose-inducible vector and analyzed the genome-wide effects on transcription and protein expression during the course of yycF expression. The induction of yycF expression increased histidine kinase yycG transcript levels, suggesting an autoregulation of the yycFG operon. Evidence from both proteomic and microarray transcriptome studies as well as analyses of membrane fatty acid composition indicated that YycFG is involved in the regulation of fatty acid biosynthesis pathways and in determining fatty acid chain lengths in membrane lipids. In agreement with recent transcriptome data on pneumococcal cells depleted of YycFG, we also identified several other potential members of the YycFG regulon that are required for virulence and cell wall biosynthesis and metabolism.

Original languageEnglish
Pages (from-to)2357-2367
Number of pages11
JournalJournal of Bacteriology
Volume187
Issue number7
DOIs
Publication statusPublished - Apr 2005
Externally publishedYes

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