Evidence of trained immunity in fish macrophages

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Trained immunity is a form of innate immune memory best described in mice and humans. Trained immunity is defined as a heightened response to a secondary infection that can be exerted toward both homologous and heterologous microorganisms. Typical criteria of trained immunity include: 1) induction upon primary infections or immunizations and subsequent protection against a secondary infection, in a T-and B-lymphocyte independent manner, 2) a response that is less specific than an adaptive immune response but that still confers increased resistance upon reinfection of the host and, 3) the involvement of innate cell types such as NK cells and macrophages involved in improved pathogen recognition and an increased inflammatory response. Clear evidence of the evolutionary conservation of trained immunity in teleost fish is lacking. Given the evolutionary position of teleosts as early vertebrates with a fully developed immune system, we hypothesize that teleost myeloid cells show features of trained immunity common to those observed in mammalian macrophages. These would at least include the ability of fish macrophages to mount heightened responses to a secondary stimulus in a non-specific manner.
We established an in vitro model to study trained immunity in fish by adapting a well-described culture system of head kidney-derived macrophages of common carp. A soluble NOD-specific ligand and a soluble β-glucan were used to train carp macrophages, after which cells were rested for six days prior to exposure to a secondary stimulus. Unstimulated trained macrophages displayed evidence ofmetabolic reprogramming, as well as heightened phagocytosis and increased expression of the inflammatory cytokines il6 and tnfα. Stimulated, trained macrophages showed heightened production of reactive oxygen and nitrogen species as compared to the corresponding stimulated but untrained cells. Thereby, trained immunity is a form of innate immune memory that appears conserved in macrophages of common carp. Measurement of the production of reactive oxygen species proved particularly informative to identify ligands able to train carp macrophages.
Original languageEnglish
Title of host publicationAbstract book of the 1st Congress of Asian Society of Developmental and Comparative Immunology (ASDCI)
Chapter102
Pages18-18
Number of pages1
Publication statusPublished - 4 Nov 2019
Event1st ASDCI Congress - Dalian Zhongxia Garden Hotel , Dalian, China
Duration: 1 Nov 20194 Nov 2019
http://asdci.dlou.edu.cn/The1stASDCICongress/list.htm

Conference

Conference1st ASDCI Congress
CountryChina
CityDalian
Period1/11/194/11/19
Internet address

Fingerprint

Immunity
Fishes
Macrophages
Carps
Coinfection
Reactive Oxygen Species
Head Kidney
Ligands
Reactive Nitrogen Species
Glucans
Adaptive Immunity
Myeloid Cells
Phagocytosis
Natural Killer Cells
Vertebrates
Immune System
Immunization
B-Lymphocytes
Cytokines
Infection

Cite this

Petit, J., Forlenza, M., & Wiegertjes, G. (2019). Evidence of trained immunity in fish macrophages. In Abstract book of the 1st Congress of Asian Society of Developmental and Comparative Immunology (ASDCI) (pp. 18-18)
Petit, J. ; Forlenza, M. ; Wiegertjes, G. / Evidence of trained immunity in fish macrophages. Abstract book of the 1st Congress of Asian Society of Developmental and Comparative Immunology (ASDCI). 2019. pp. 18-18
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Petit, J, Forlenza, M & Wiegertjes, G 2019, Evidence of trained immunity in fish macrophages. in Abstract book of the 1st Congress of Asian Society of Developmental and Comparative Immunology (ASDCI). pp. 18-18, 1st ASDCI Congress, Dalian, China, 1/11/19.

Evidence of trained immunity in fish macrophages. / Petit, J.; Forlenza, M.; Wiegertjes, G.

Abstract book of the 1st Congress of Asian Society of Developmental and Comparative Immunology (ASDCI). 2019. p. 18-18.

Research output: Chapter in Book/Report/Conference proceedingAbstract

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N2 - Trained immunity is a form of innate immune memory best described in mice and humans. Trained immunity is defined as a heightened response to a secondary infection that can be exerted toward both homologous and heterologous microorganisms. Typical criteria of trained immunity include: 1) induction upon primary infections or immunizations and subsequent protection against a secondary infection, in a T-and B-lymphocyte independent manner, 2) a response that is less specific than an adaptive immune response but that still confers increased resistance upon reinfection of the host and, 3) the involvement of innate cell types such as NK cells and macrophages involved in improved pathogen recognition and an increased inflammatory response. Clear evidence of the evolutionary conservation of trained immunity in teleost fish is lacking. Given the evolutionary position of teleosts as early vertebrates with a fully developed immune system, we hypothesize that teleost myeloid cells show features of trained immunity common to those observed in mammalian macrophages. These would at least include the ability of fish macrophages to mount heightened responses to a secondary stimulus in a non-specific manner.We established an in vitro model to study trained immunity in fish by adapting a well-described culture system of head kidney-derived macrophages of common carp. A soluble NOD-specific ligand and a soluble β-glucan were used to train carp macrophages, after which cells were rested for six days prior to exposure to a secondary stimulus. Unstimulated trained macrophages displayed evidence ofmetabolic reprogramming, as well as heightened phagocytosis and increased expression of the inflammatory cytokines il6 and tnfα. Stimulated, trained macrophages showed heightened production of reactive oxygen and nitrogen species as compared to the corresponding stimulated but untrained cells. Thereby, trained immunity is a form of innate immune memory that appears conserved in macrophages of common carp. Measurement of the production of reactive oxygen species proved particularly informative to identify ligands able to train carp macrophages.

AB - Trained immunity is a form of innate immune memory best described in mice and humans. Trained immunity is defined as a heightened response to a secondary infection that can be exerted toward both homologous and heterologous microorganisms. Typical criteria of trained immunity include: 1) induction upon primary infections or immunizations and subsequent protection against a secondary infection, in a T-and B-lymphocyte independent manner, 2) a response that is less specific than an adaptive immune response but that still confers increased resistance upon reinfection of the host and, 3) the involvement of innate cell types such as NK cells and macrophages involved in improved pathogen recognition and an increased inflammatory response. Clear evidence of the evolutionary conservation of trained immunity in teleost fish is lacking. Given the evolutionary position of teleosts as early vertebrates with a fully developed immune system, we hypothesize that teleost myeloid cells show features of trained immunity common to those observed in mammalian macrophages. These would at least include the ability of fish macrophages to mount heightened responses to a secondary stimulus in a non-specific manner.We established an in vitro model to study trained immunity in fish by adapting a well-described culture system of head kidney-derived macrophages of common carp. A soluble NOD-specific ligand and a soluble β-glucan were used to train carp macrophages, after which cells were rested for six days prior to exposure to a secondary stimulus. Unstimulated trained macrophages displayed evidence ofmetabolic reprogramming, as well as heightened phagocytosis and increased expression of the inflammatory cytokines il6 and tnfα. Stimulated, trained macrophages showed heightened production of reactive oxygen and nitrogen species as compared to the corresponding stimulated but untrained cells. Thereby, trained immunity is a form of innate immune memory that appears conserved in macrophages of common carp. Measurement of the production of reactive oxygen species proved particularly informative to identify ligands able to train carp macrophages.

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ER -

Petit J, Forlenza M, Wiegertjes G. Evidence of trained immunity in fish macrophages. In Abstract book of the 1st Congress of Asian Society of Developmental and Comparative Immunology (ASDCI). 2019. p. 18-18