Evidence for rapid evolution in a grassland biodiversity experiment

Sofia J. van Moorsel*, Marc W. Schmid, Niels C.A.M. Wagemaker, Thomas van Gurp, Bernhard Schmid, Philippine Vergeer

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

In long-term grassland experiments, positive biodiversity effects on plant productivity commonly increase with time. Subsequent glasshouse experiments showed that these strengthened positive biodiversity effects persist not only in the local environment but also when plants are transferred into a common environment. Thus, we hypothesized that community diversity had acted as a selective agent, resulting in the emergence of plant monoculture and mixture types with differing genetic composition. To test our hypothesis, we grew offspring from plants that were grown for eleven years in monoculture or mixture environments in a biodiversity experiment (Jena Experiment) under controlled glasshouse conditions in monocultures or two-species mixtures. We used epiGBS, a genotyping-by-sequencing approach combined with bisulphite conversion, to provide integrative genetic and epigenetic (i.e., DNA methylation) data. We observed significant divergence in genetic and DNA methylation data according to selection history in three out of five perennial grassland species, namely Galium mollugo, Prunella vulgaris and Veronica chamaedrys, with DNA methylation differences mostly reflecting the genetic differences. In addition, current diversity levels in the glasshouse had weak effects on epigenetic variation. However, given the limited genome coverage of the reference-free bisulphite method epiGBS, it remains unclear how much of the differences in DNA methylation was independent of underlying genetic differences. Our results thus suggest that selection of genetic variants, and possibly epigenetic variants, caused the rapid emergence of monoculture and mixture types within plant species in the Jena Experiment.

Original languageEnglish
Pages (from-to)4097-4117
JournalMolecular Ecology
Volume28
Issue number17
DOIs
Publication statusPublished - Sep 2019

Fingerprint

Biodiversity
DNA methylation
DNA Methylation
grasslands
grassland
methylation
biodiversity
monoculture
Epigenomics
epigenetics
bisulfites
DNA
greenhouses
experiment
Molluginaceae
Veronica chamaedrys
Prunella
Galium mollugo
Veronica
Galium

Keywords

  • biodiversity
  • DNA methylation
  • epigenetic variation
  • genetic divergence
  • herbaceous plant species
  • selection

Cite this

van Moorsel, S. J., Schmid, M. W., Wagemaker, N. C. A. M., van Gurp, T., Schmid, B., & Vergeer, P. (2019). Evidence for rapid evolution in a grassland biodiversity experiment. Molecular Ecology, 28(17), 4097-4117. https://doi.org/10.1111/mec.15191
van Moorsel, Sofia J. ; Schmid, Marc W. ; Wagemaker, Niels C.A.M. ; van Gurp, Thomas ; Schmid, Bernhard ; Vergeer, Philippine. / Evidence for rapid evolution in a grassland biodiversity experiment. In: Molecular Ecology. 2019 ; Vol. 28, No. 17. pp. 4097-4117.
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van Moorsel, SJ, Schmid, MW, Wagemaker, NCAM, van Gurp, T, Schmid, B & Vergeer, P 2019, 'Evidence for rapid evolution in a grassland biodiversity experiment', Molecular Ecology, vol. 28, no. 17, pp. 4097-4117. https://doi.org/10.1111/mec.15191

Evidence for rapid evolution in a grassland biodiversity experiment. / van Moorsel, Sofia J.; Schmid, Marc W.; Wagemaker, Niels C.A.M.; van Gurp, Thomas; Schmid, Bernhard; Vergeer, Philippine.

In: Molecular Ecology, Vol. 28, No. 17, 09.2019, p. 4097-4117.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - van Moorsel, Sofia J.

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AU - Wagemaker, Niels C.A.M.

AU - van Gurp, Thomas

AU - Schmid, Bernhard

AU - Vergeer, Philippine

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AB - In long-term grassland experiments, positive biodiversity effects on plant productivity commonly increase with time. Subsequent glasshouse experiments showed that these strengthened positive biodiversity effects persist not only in the local environment but also when plants are transferred into a common environment. Thus, we hypothesized that community diversity had acted as a selective agent, resulting in the emergence of plant monoculture and mixture types with differing genetic composition. To test our hypothesis, we grew offspring from plants that were grown for eleven years in monoculture or mixture environments in a biodiversity experiment (Jena Experiment) under controlled glasshouse conditions in monocultures or two-species mixtures. We used epiGBS, a genotyping-by-sequencing approach combined with bisulphite conversion, to provide integrative genetic and epigenetic (i.e., DNA methylation) data. We observed significant divergence in genetic and DNA methylation data according to selection history in three out of five perennial grassland species, namely Galium mollugo, Prunella vulgaris and Veronica chamaedrys, with DNA methylation differences mostly reflecting the genetic differences. In addition, current diversity levels in the glasshouse had weak effects on epigenetic variation. However, given the limited genome coverage of the reference-free bisulphite method epiGBS, it remains unclear how much of the differences in DNA methylation was independent of underlying genetic differences. Our results thus suggest that selection of genetic variants, and possibly epigenetic variants, caused the rapid emergence of monoculture and mixture types within plant species in the Jena Experiment.

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JO - Molecular Ecology

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van Moorsel SJ, Schmid MW, Wagemaker NCAM, van Gurp T, Schmid B, Vergeer P. Evidence for rapid evolution in a grassland biodiversity experiment. Molecular Ecology. 2019 Sep;28(17):4097-4117. https://doi.org/10.1111/mec.15191