Evidence for natural selection in the mitochondrial genome of Mycosphaerella graminicola

J. Zhan, G.H.J. Kema

    Research output: Contribution to journalArticleAcademicpeer-review

    20 Citations (Scopus)

    Abstract

    Pathogenicity assays were combined with restriction fragment length polymorphism (RFLP) markers in the mitochondrial and nuclear genomes to compare Mycosphaerella grominicola populations adapted to bread wheat (Triticum aestivum) and durum wheat (T. turgidum) in the Mediterranean Basin. The majority of isolates had unique nuclear DNA fingerprints and multilocus haplotypes. Only six mitochondrial DNA (mtDNA) haplotypes were identified among 108 isolates assayed. There were minor differences in frequencies of alleles at nuclear RFLP loci between the two host-adapted populations, but differences in the frequencies of mtDNA haplotypes were highly significant (P <0.0001). mtDNA haplotype I dominated on the isolates adapted to bread wheat, and its frequency was twice as high as for the isolates adapted to durum wheat. mtDNA haplotype 4. which contained a unique approximate to3-kb insertion, was detected only in isolates showing specificity toward durum wheat and was the dominant haplotype on this species. We propose that the low mitochondrial diversity in this pathogenic fungus is due to a selective sweep and that differences in the frequencies of mtDNA haplotypes between the two host-adapted populations, were due to natural selection according, to host species.
    Original languageEnglish
    Pages (from-to)261-267
    JournalPhytopathology
    Volume94
    Issue number3
    DOIs
    Publication statusPublished - 2004

    Fingerprint

    Mycosphaerella graminicola
    natural selection
    haplotypes
    mitochondrial DNA
    durum wheat
    nuclear genome
    restriction fragment length polymorphism
    Mycosphaerella
    wheat
    Triticum turgidum
    Triticum turgidum subsp. durum
    mitochondrial genome
    DNA fingerprinting
    gene frequency
    pathogenicity
    Triticum aestivum
    basins
    loci
    fungi
    assays

    Keywords

    • fragment-length-polymorphisms
    • anamorph-septoria-tritici
    • in-field populations
    • genetic-structure
    • sexual reproduction
    • cryphonectria-parasitica
    • stagonospora-nodorum
    • pyricularia-grisea
    • agaricus-bisporus
    • host-specificity

    Cite this

    @article{b6e045af97b1446badd3ec925696c6b1,
    title = "Evidence for natural selection in the mitochondrial genome of Mycosphaerella graminicola",
    abstract = "Pathogenicity assays were combined with restriction fragment length polymorphism (RFLP) markers in the mitochondrial and nuclear genomes to compare Mycosphaerella grominicola populations adapted to bread wheat (Triticum aestivum) and durum wheat (T. turgidum) in the Mediterranean Basin. The majority of isolates had unique nuclear DNA fingerprints and multilocus haplotypes. Only six mitochondrial DNA (mtDNA) haplotypes were identified among 108 isolates assayed. There were minor differences in frequencies of alleles at nuclear RFLP loci between the two host-adapted populations, but differences in the frequencies of mtDNA haplotypes were highly significant (P <0.0001). mtDNA haplotype I dominated on the isolates adapted to bread wheat, and its frequency was twice as high as for the isolates adapted to durum wheat. mtDNA haplotype 4. which contained a unique approximate to3-kb insertion, was detected only in isolates showing specificity toward durum wheat and was the dominant haplotype on this species. We propose that the low mitochondrial diversity in this pathogenic fungus is due to a selective sweep and that differences in the frequencies of mtDNA haplotypes between the two host-adapted populations, were due to natural selection according, to host species.",
    keywords = "fragment-length-polymorphisms, anamorph-septoria-tritici, in-field populations, genetic-structure, sexual reproduction, cryphonectria-parasitica, stagonospora-nodorum, pyricularia-grisea, agaricus-bisporus, host-specificity",
    author = "J. Zhan and G.H.J. Kema",
    note = "'Applied Mycology & Biotechnology' is an International Series. Titel of Volume 4: Fungal Genomics. Elsevier Science B.",
    year = "2004",
    doi = "10.1094/PHYTO.2004.94.3.261",
    language = "English",
    volume = "94",
    pages = "261--267",
    journal = "Phytopathology",
    issn = "0031-949X",
    publisher = "American Phytopathological Society",
    number = "3",

    }

    Evidence for natural selection in the mitochondrial genome of Mycosphaerella graminicola. / Zhan, J.; Kema, G.H.J.

    In: Phytopathology, Vol. 94, No. 3, 2004, p. 261-267.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Evidence for natural selection in the mitochondrial genome of Mycosphaerella graminicola

    AU - Zhan, J.

    AU - Kema, G.H.J.

    N1 - 'Applied Mycology & Biotechnology' is an International Series. Titel of Volume 4: Fungal Genomics. Elsevier Science B.

    PY - 2004

    Y1 - 2004

    N2 - Pathogenicity assays were combined with restriction fragment length polymorphism (RFLP) markers in the mitochondrial and nuclear genomes to compare Mycosphaerella grominicola populations adapted to bread wheat (Triticum aestivum) and durum wheat (T. turgidum) in the Mediterranean Basin. The majority of isolates had unique nuclear DNA fingerprints and multilocus haplotypes. Only six mitochondrial DNA (mtDNA) haplotypes were identified among 108 isolates assayed. There were minor differences in frequencies of alleles at nuclear RFLP loci between the two host-adapted populations, but differences in the frequencies of mtDNA haplotypes were highly significant (P <0.0001). mtDNA haplotype I dominated on the isolates adapted to bread wheat, and its frequency was twice as high as for the isolates adapted to durum wheat. mtDNA haplotype 4. which contained a unique approximate to3-kb insertion, was detected only in isolates showing specificity toward durum wheat and was the dominant haplotype on this species. We propose that the low mitochondrial diversity in this pathogenic fungus is due to a selective sweep and that differences in the frequencies of mtDNA haplotypes between the two host-adapted populations, were due to natural selection according, to host species.

    AB - Pathogenicity assays were combined with restriction fragment length polymorphism (RFLP) markers in the mitochondrial and nuclear genomes to compare Mycosphaerella grominicola populations adapted to bread wheat (Triticum aestivum) and durum wheat (T. turgidum) in the Mediterranean Basin. The majority of isolates had unique nuclear DNA fingerprints and multilocus haplotypes. Only six mitochondrial DNA (mtDNA) haplotypes were identified among 108 isolates assayed. There were minor differences in frequencies of alleles at nuclear RFLP loci between the two host-adapted populations, but differences in the frequencies of mtDNA haplotypes were highly significant (P <0.0001). mtDNA haplotype I dominated on the isolates adapted to bread wheat, and its frequency was twice as high as for the isolates adapted to durum wheat. mtDNA haplotype 4. which contained a unique approximate to3-kb insertion, was detected only in isolates showing specificity toward durum wheat and was the dominant haplotype on this species. We propose that the low mitochondrial diversity in this pathogenic fungus is due to a selective sweep and that differences in the frequencies of mtDNA haplotypes between the two host-adapted populations, were due to natural selection according, to host species.

    KW - fragment-length-polymorphisms

    KW - anamorph-septoria-tritici

    KW - in-field populations

    KW - genetic-structure

    KW - sexual reproduction

    KW - cryphonectria-parasitica

    KW - stagonospora-nodorum

    KW - pyricularia-grisea

    KW - agaricus-bisporus

    KW - host-specificity

    U2 - 10.1094/PHYTO.2004.94.3.261

    DO - 10.1094/PHYTO.2004.94.3.261

    M3 - Article

    VL - 94

    SP - 261

    EP - 267

    JO - Phytopathology

    JF - Phytopathology

    SN - 0031-949X

    IS - 3

    ER -