Evidence for a role of CCK as neurotransmitter in the guinea-pig enteric nervous system.

I.W.M. Schutte; K.B.C.W. Hollestein; L.M.A. Akkermans; A.B.A. Kroese

doi:10.1016/S0304-3940(97)00786-6

Evidence for a role of CCK as neurotransmitter in the guinea-pig enteric nervous system.

I.W.M. Schutte, K.B.C.W. Hollestein, L.M.A. Akkermans, A.B.A. Kroese

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Intracellular recordings were made of neurons in the myenteric plexus of the guinea-pig distal ileum. Slow excitatory post-synaptic potentials (sEPSPs) were evoked by electrical stimulation of an interganglionic fibre tract. The effect of cholecystokinin (CCK) receptor antagonists on the sEPSPs was investigated in 11 neurons. Application of the CCK receptor antagonists L-364,718 and L-365.260 (each 250 nM) markedly attenuated the sEPSPs in five of 11 neurons. The amplitude of the sEPSP reduced from 15 ± 3 to 7 ± 2 mV and the change in membrane resistance during the sEPSP was reduced from 28 ± 9 to 11 ± 8 MΩ. In six of 11 neurons the CCK antagonists had no effect on the sEPSPs. The results provide evidence that neurally released CCK is involved in the mediation of sEPSPs in some enteric neurons.

Original language	English
Pages (from-to)	155-158
Journal	Neuroscience Letters
Volume	236
DOIs	https://doi.org/10.1016/S0304-3940(97)00786-6
Publication status	Published - 1997

Keywords

Antagonists
Cholecystokinin
Distal ileum
Enteric neurons
Neuropeptides
Slow exitatory postsynaptic potential

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10.1016/S0304-3940(97)00786-6

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title = "Evidence for a role of CCK as neurotransmitter in the guinea-pig enteric nervous system.",

abstract = "Intracellular recordings were made of neurons in the myenteric plexus of the guinea-pig distal ileum. Slow excitatory post-synaptic potentials (sEPSPs) were evoked by electrical stimulation of an interganglionic fibre tract. The effect of cholecystokinin (CCK) receptor antagonists on the sEPSPs was investigated in 11 neurons. Application of the CCK receptor antagonists L-364,718 and L-365.260 (each 250 nM) markedly attenuated the sEPSPs in five of 11 neurons. The amplitude of the sEPSP reduced from 15 ± 3 to 7 ± 2 mV and the change in membrane resistance during the sEPSP was reduced from 28 ± 9 to 11 ± 8 MΩ. In six of 11 neurons the CCK antagonists had no effect on the sEPSPs. The results provide evidence that neurally released CCK is involved in the mediation of sEPSPs in some enteric neurons.",

keywords = "Antagonists, Cholecystokinin, Distal ileum, Enteric neurons, Neuropeptides, Slow exitatory postsynaptic potential",

author = "I.W.M. Schutte and K.B.C.W. Hollestein and L.M.A. Akkermans and A.B.A. Kroese",

year = "1997",

doi = "10.1016/S0304-3940(97)00786-6",

language = "English",

volume = "236",

pages = "155--158",

journal = "Neuroscience Letters",

issn = "0304-3940",

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TY - JOUR

T1 - Evidence for a role of CCK as neurotransmitter in the guinea-pig enteric nervous system.

AU - Schutte, I.W.M.

AU - Hollestein, K.B.C.W.

AU - Akkermans, L.M.A.

AU - Kroese, A.B.A.

PY - 1997

Y1 - 1997

N2 - Intracellular recordings were made of neurons in the myenteric plexus of the guinea-pig distal ileum. Slow excitatory post-synaptic potentials (sEPSPs) were evoked by electrical stimulation of an interganglionic fibre tract. The effect of cholecystokinin (CCK) receptor antagonists on the sEPSPs was investigated in 11 neurons. Application of the CCK receptor antagonists L-364,718 and L-365.260 (each 250 nM) markedly attenuated the sEPSPs in five of 11 neurons. The amplitude of the sEPSP reduced from 15 ± 3 to 7 ± 2 mV and the change in membrane resistance during the sEPSP was reduced from 28 ± 9 to 11 ± 8 MΩ. In six of 11 neurons the CCK antagonists had no effect on the sEPSPs. The results provide evidence that neurally released CCK is involved in the mediation of sEPSPs in some enteric neurons.

AB - Intracellular recordings were made of neurons in the myenteric plexus of the guinea-pig distal ileum. Slow excitatory post-synaptic potentials (sEPSPs) were evoked by electrical stimulation of an interganglionic fibre tract. The effect of cholecystokinin (CCK) receptor antagonists on the sEPSPs was investigated in 11 neurons. Application of the CCK receptor antagonists L-364,718 and L-365.260 (each 250 nM) markedly attenuated the sEPSPs in five of 11 neurons. The amplitude of the sEPSP reduced from 15 ± 3 to 7 ± 2 mV and the change in membrane resistance during the sEPSP was reduced from 28 ± 9 to 11 ± 8 MΩ. In six of 11 neurons the CCK antagonists had no effect on the sEPSPs. The results provide evidence that neurally released CCK is involved in the mediation of sEPSPs in some enteric neurons.

KW - Antagonists

KW - Cholecystokinin

KW - Distal ileum

KW - Enteric neurons

KW - Neuropeptides

KW - Slow exitatory postsynaptic potential

U2 - 10.1016/S0304-3940(97)00786-6

DO - 10.1016/S0304-3940(97)00786-6

M3 - Article

SN - 0304-3940

VL - 236

SP - 155

EP - 158

JO - Neuroscience Letters

JF - Neuroscience Letters

ER -

Evidence for a role of CCK as neurotransmitter in the guinea-pig enteric nervous system.

Abstract

Keywords

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