Evaluation of the use of animal compounds as substrates by felid fecal microbiota: a cheetah model

S. Depauw, M. Hesta, G.P.J. Janssens, G. Bosch

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

Although strict carnivores like felids do not consume plant fibre, considerable intestinal fermentation has been measured. This indicates that certain animal-derived compounds can serve as substrates for fermentation and microbial proliferation in the feline hindgut. It is often claimed that enzymatically indigested animal material exerts the production of toxic compounds in the hindgut, but to date, only little peer-reviewed documentation is available, whereas in contrast, recent findings point to specific microorganisms in the feline hindgut that use proteolysis products as a substrate. The present study investigated to which extent faecal microbiota of the cheetah (as model for strict carnivores) ferment animal tissues and which would be the concomitant end product profile. Fresh faecal samples of captive cheetahs were collected and processed into an inoculum, combined with following substrates: casein (pure protein), bone, skin, hair, cartilage, collagen, chondroitin-glucosamine mixture, glucosamine, fructo-oligosaccharides (FOS, positive control), cellulose (negative control). During 72 hours, cumulative gas production was continuously registered. Thereafter, incubates were sampled for analysis of volatile fatty acids (VFA). Gas production was highest for FOS, followed by glucosamine, chondroitin-glucosamine mixture and cartilage. Casein gave intermediate gas production, but still more than collagen. Bone, skin, hair and cellulose were poor fermentation substrates. Interestingly, maximum gas production rate of FOS occurred much later compared to all animal substrates. VFA production was highest with FOS. Glucosamine and glucosamine-chondroitin showed high VFA production, whereas cartilage and casein produced moderate VFA. Despite the low gas production, collagen had high short-chain fatty acids production and showed, in contrast to all other substrates, a very high ratio of acetic to propionic acid. The present data indicate that cartilage and cartilage related compounds are well-fermentable animal tissue for faecal microbiota of the cheetah. Moreover, collagen can also contribute to microbial fermentation, but behaves differently and needs to be look at thoroughly.
Original languageEnglish
Title of host publicationThe Waltham International Nutritional Sciences Symposium; Pet Nutrition- Art or Science?, Cambridge, UK, 16 - 19 September, 2010
Place of PublicationMelton Mowbray
PublisherWaltham Centre for Pet Nutrition
Pages117-117
Publication statusPublished - 2010
EventThe Waltham International Nutritional Sciences Symposium; Pet Nutrition- Art or Science?, Cambridge, UK -
Duration: 16 Sep 201019 Sep 2010

Conference

ConferenceThe Waltham International Nutritional Sciences Symposium; Pet Nutrition- Art or Science?, Cambridge, UK
Period16/09/1019/09/10

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Acinonyx jubatus
Felidae
glucosamine
gas production (biological)
cartilage
volatile fatty acids
collagen
hindgut
fermentation
casein
animals
animal tissues
skin (animal)
carnivores
hairs
cellulose
bones
cats
plant fibers
fructooligosaccharides

Cite this

Depauw, S., Hesta, M., Janssens, G. P. J., & Bosch, G. (2010). Evaluation of the use of animal compounds as substrates by felid fecal microbiota: a cheetah model. In The Waltham International Nutritional Sciences Symposium; Pet Nutrition- Art or Science?, Cambridge, UK, 16 - 19 September, 2010 (pp. 117-117). Melton Mowbray: Waltham Centre for Pet Nutrition.
Depauw, S. ; Hesta, M. ; Janssens, G.P.J. ; Bosch, G. / Evaluation of the use of animal compounds as substrates by felid fecal microbiota: a cheetah model. The Waltham International Nutritional Sciences Symposium; Pet Nutrition- Art or Science?, Cambridge, UK, 16 - 19 September, 2010. Melton Mowbray : Waltham Centre for Pet Nutrition, 2010. pp. 117-117
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abstract = "Although strict carnivores like felids do not consume plant fibre, considerable intestinal fermentation has been measured. This indicates that certain animal-derived compounds can serve as substrates for fermentation and microbial proliferation in the feline hindgut. It is often claimed that enzymatically indigested animal material exerts the production of toxic compounds in the hindgut, but to date, only little peer-reviewed documentation is available, whereas in contrast, recent findings point to specific microorganisms in the feline hindgut that use proteolysis products as a substrate. The present study investigated to which extent faecal microbiota of the cheetah (as model for strict carnivores) ferment animal tissues and which would be the concomitant end product profile. Fresh faecal samples of captive cheetahs were collected and processed into an inoculum, combined with following substrates: casein (pure protein), bone, skin, hair, cartilage, collagen, chondroitin-glucosamine mixture, glucosamine, fructo-oligosaccharides (FOS, positive control), cellulose (negative control). During 72 hours, cumulative gas production was continuously registered. Thereafter, incubates were sampled for analysis of volatile fatty acids (VFA). Gas production was highest for FOS, followed by glucosamine, chondroitin-glucosamine mixture and cartilage. Casein gave intermediate gas production, but still more than collagen. Bone, skin, hair and cellulose were poor fermentation substrates. Interestingly, maximum gas production rate of FOS occurred much later compared to all animal substrates. VFA production was highest with FOS. Glucosamine and glucosamine-chondroitin showed high VFA production, whereas cartilage and casein produced moderate VFA. Despite the low gas production, collagen had high short-chain fatty acids production and showed, in contrast to all other substrates, a very high ratio of acetic to propionic acid. The present data indicate that cartilage and cartilage related compounds are well-fermentable animal tissue for faecal microbiota of the cheetah. Moreover, collagen can also contribute to microbial fermentation, but behaves differently and needs to be look at thoroughly.",
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Depauw, S, Hesta, M, Janssens, GPJ & Bosch, G 2010, Evaluation of the use of animal compounds as substrates by felid fecal microbiota: a cheetah model. in The Waltham International Nutritional Sciences Symposium; Pet Nutrition- Art or Science?, Cambridge, UK, 16 - 19 September, 2010. Waltham Centre for Pet Nutrition, Melton Mowbray, pp. 117-117, The Waltham International Nutritional Sciences Symposium; Pet Nutrition- Art or Science?, Cambridge, UK, 16/09/10.

Evaluation of the use of animal compounds as substrates by felid fecal microbiota: a cheetah model. / Depauw, S.; Hesta, M.; Janssens, G.P.J.; Bosch, G.

The Waltham International Nutritional Sciences Symposium; Pet Nutrition- Art or Science?, Cambridge, UK, 16 - 19 September, 2010. Melton Mowbray : Waltham Centre for Pet Nutrition, 2010. p. 117-117.

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

TY - CHAP

T1 - Evaluation of the use of animal compounds as substrates by felid fecal microbiota: a cheetah model

AU - Depauw, S.

AU - Hesta, M.

AU - Janssens, G.P.J.

AU - Bosch, G.

PY - 2010

Y1 - 2010

N2 - Although strict carnivores like felids do not consume plant fibre, considerable intestinal fermentation has been measured. This indicates that certain animal-derived compounds can serve as substrates for fermentation and microbial proliferation in the feline hindgut. It is often claimed that enzymatically indigested animal material exerts the production of toxic compounds in the hindgut, but to date, only little peer-reviewed documentation is available, whereas in contrast, recent findings point to specific microorganisms in the feline hindgut that use proteolysis products as a substrate. The present study investigated to which extent faecal microbiota of the cheetah (as model for strict carnivores) ferment animal tissues and which would be the concomitant end product profile. Fresh faecal samples of captive cheetahs were collected and processed into an inoculum, combined with following substrates: casein (pure protein), bone, skin, hair, cartilage, collagen, chondroitin-glucosamine mixture, glucosamine, fructo-oligosaccharides (FOS, positive control), cellulose (negative control). During 72 hours, cumulative gas production was continuously registered. Thereafter, incubates were sampled for analysis of volatile fatty acids (VFA). Gas production was highest for FOS, followed by glucosamine, chondroitin-glucosamine mixture and cartilage. Casein gave intermediate gas production, but still more than collagen. Bone, skin, hair and cellulose were poor fermentation substrates. Interestingly, maximum gas production rate of FOS occurred much later compared to all animal substrates. VFA production was highest with FOS. Glucosamine and glucosamine-chondroitin showed high VFA production, whereas cartilage and casein produced moderate VFA. Despite the low gas production, collagen had high short-chain fatty acids production and showed, in contrast to all other substrates, a very high ratio of acetic to propionic acid. The present data indicate that cartilage and cartilage related compounds are well-fermentable animal tissue for faecal microbiota of the cheetah. Moreover, collagen can also contribute to microbial fermentation, but behaves differently and needs to be look at thoroughly.

AB - Although strict carnivores like felids do not consume plant fibre, considerable intestinal fermentation has been measured. This indicates that certain animal-derived compounds can serve as substrates for fermentation and microbial proliferation in the feline hindgut. It is often claimed that enzymatically indigested animal material exerts the production of toxic compounds in the hindgut, but to date, only little peer-reviewed documentation is available, whereas in contrast, recent findings point to specific microorganisms in the feline hindgut that use proteolysis products as a substrate. The present study investigated to which extent faecal microbiota of the cheetah (as model for strict carnivores) ferment animal tissues and which would be the concomitant end product profile. Fresh faecal samples of captive cheetahs were collected and processed into an inoculum, combined with following substrates: casein (pure protein), bone, skin, hair, cartilage, collagen, chondroitin-glucosamine mixture, glucosamine, fructo-oligosaccharides (FOS, positive control), cellulose (negative control). During 72 hours, cumulative gas production was continuously registered. Thereafter, incubates were sampled for analysis of volatile fatty acids (VFA). Gas production was highest for FOS, followed by glucosamine, chondroitin-glucosamine mixture and cartilage. Casein gave intermediate gas production, but still more than collagen. Bone, skin, hair and cellulose were poor fermentation substrates. Interestingly, maximum gas production rate of FOS occurred much later compared to all animal substrates. VFA production was highest with FOS. Glucosamine and glucosamine-chondroitin showed high VFA production, whereas cartilage and casein produced moderate VFA. Despite the low gas production, collagen had high short-chain fatty acids production and showed, in contrast to all other substrates, a very high ratio of acetic to propionic acid. The present data indicate that cartilage and cartilage related compounds are well-fermentable animal tissue for faecal microbiota of the cheetah. Moreover, collagen can also contribute to microbial fermentation, but behaves differently and needs to be look at thoroughly.

M3 - Abstract

SP - 117

EP - 117

BT - The Waltham International Nutritional Sciences Symposium; Pet Nutrition- Art or Science?, Cambridge, UK, 16 - 19 September, 2010

PB - Waltham Centre for Pet Nutrition

CY - Melton Mowbray

ER -

Depauw S, Hesta M, Janssens GPJ, Bosch G. Evaluation of the use of animal compounds as substrates by felid fecal microbiota: a cheetah model. In The Waltham International Nutritional Sciences Symposium; Pet Nutrition- Art or Science?, Cambridge, UK, 16 - 19 September, 2010. Melton Mowbray: Waltham Centre for Pet Nutrition. 2010. p. 117-117