Evaluation of the Discriminative Potential of a Novel Biomarker for Estradiol Treatments in Bovine Animals

P. Regal, M.H. Blokland, C.A. Fente, S.S. Sterk, A. Cepeda, L.A. van Ginkel

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

The endogenous occurrence of natural hormones obstructs the application of classical targeted methods as confirmatory options. In the case of estradiol, the ultimate confirmation of its exogenous administration relies on gas chromatography coupled to combustion/isotope ratio mass spectrometry (GC-C/IRMS). A serum dipeptide composed of pyroglutamic acid and phenylalanine was identified as a potential biomarker of estradiol treatments in adult cows. To evaluate its potential to pinpoint suspicious samples, samples from prepubertal females under different estrogenic treatments have been analyzed. The results confirmed the up-regulation of the dipeptide in adult bovines. The 2-week-old females exhibited short-lasting responses only in a few animals. The 6-month-old female showed a delayed but clear increase on the biomarker level. The composition of the anabolic preparations, the dose, and/or the administration route are possible additional reasons for the reduced response in young animals. A comparison to previous results reported by various researchers is included.
Original languageEnglish
Pages (from-to)370-378
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number1
DOIs
Publication statusPublished - 2015

Fingerprint

Dipeptides
Biomarkers
estradiol
Estradiol
biomarkers
Animals
dipeptides
Pyrrolidonecarboxylic Acid
cattle
Phenylalanine
Isotopes
Gas chromatography
Mass spectrometry
animals
young animals
Hormones
combustion
phenylalanine
Gas Chromatography
isotopes

Keywords

  • tandem mass-spectrometry
  • timed artificial-insemination
  • beef-cattle
  • protein biomarkers
  • gene-expression
  • hormone abuse
  • plasma
  • 17-beta-estradiol
  • estrogen
  • detect

Cite this

@article{34264bd201844432be3f99ae0d884508,
title = "Evaluation of the Discriminative Potential of a Novel Biomarker for Estradiol Treatments in Bovine Animals",
abstract = "The endogenous occurrence of natural hormones obstructs the application of classical targeted methods as confirmatory options. In the case of estradiol, the ultimate confirmation of its exogenous administration relies on gas chromatography coupled to combustion/isotope ratio mass spectrometry (GC-C/IRMS). A serum dipeptide composed of pyroglutamic acid and phenylalanine was identified as a potential biomarker of estradiol treatments in adult cows. To evaluate its potential to pinpoint suspicious samples, samples from prepubertal females under different estrogenic treatments have been analyzed. The results confirmed the up-regulation of the dipeptide in adult bovines. The 2-week-old females exhibited short-lasting responses only in a few animals. The 6-month-old female showed a delayed but clear increase on the biomarker level. The composition of the anabolic preparations, the dose, and/or the administration route are possible additional reasons for the reduced response in young animals. A comparison to previous results reported by various researchers is included.",
keywords = "tandem mass-spectrometry, timed artificial-insemination, beef-cattle, protein biomarkers, gene-expression, hormone abuse, plasma, 17-beta-estradiol, estrogen, detect",
author = "P. Regal and M.H. Blokland and C.A. Fente and S.S. Sterk and A. Cepeda and {van Ginkel}, L.A.",
year = "2015",
doi = "10.1021/jf503773u",
language = "English",
volume = "63",
pages = "370--378",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "1",

}

Evaluation of the Discriminative Potential of a Novel Biomarker for Estradiol Treatments in Bovine Animals. / Regal, P.; Blokland, M.H.; Fente, C.A.; Sterk, S.S.; Cepeda, A.; van Ginkel, L.A.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 1, 2015, p. 370-378.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Evaluation of the Discriminative Potential of a Novel Biomarker for Estradiol Treatments in Bovine Animals

AU - Regal, P.

AU - Blokland, M.H.

AU - Fente, C.A.

AU - Sterk, S.S.

AU - Cepeda, A.

AU - van Ginkel, L.A.

PY - 2015

Y1 - 2015

N2 - The endogenous occurrence of natural hormones obstructs the application of classical targeted methods as confirmatory options. In the case of estradiol, the ultimate confirmation of its exogenous administration relies on gas chromatography coupled to combustion/isotope ratio mass spectrometry (GC-C/IRMS). A serum dipeptide composed of pyroglutamic acid and phenylalanine was identified as a potential biomarker of estradiol treatments in adult cows. To evaluate its potential to pinpoint suspicious samples, samples from prepubertal females under different estrogenic treatments have been analyzed. The results confirmed the up-regulation of the dipeptide in adult bovines. The 2-week-old females exhibited short-lasting responses only in a few animals. The 6-month-old female showed a delayed but clear increase on the biomarker level. The composition of the anabolic preparations, the dose, and/or the administration route are possible additional reasons for the reduced response in young animals. A comparison to previous results reported by various researchers is included.

AB - The endogenous occurrence of natural hormones obstructs the application of classical targeted methods as confirmatory options. In the case of estradiol, the ultimate confirmation of its exogenous administration relies on gas chromatography coupled to combustion/isotope ratio mass spectrometry (GC-C/IRMS). A serum dipeptide composed of pyroglutamic acid and phenylalanine was identified as a potential biomarker of estradiol treatments in adult cows. To evaluate its potential to pinpoint suspicious samples, samples from prepubertal females under different estrogenic treatments have been analyzed. The results confirmed the up-regulation of the dipeptide in adult bovines. The 2-week-old females exhibited short-lasting responses only in a few animals. The 6-month-old female showed a delayed but clear increase on the biomarker level. The composition of the anabolic preparations, the dose, and/or the administration route are possible additional reasons for the reduced response in young animals. A comparison to previous results reported by various researchers is included.

KW - tandem mass-spectrometry

KW - timed artificial-insemination

KW - beef-cattle

KW - protein biomarkers

KW - gene-expression

KW - hormone abuse

KW - plasma

KW - 17-beta-estradiol

KW - estrogen

KW - detect

U2 - 10.1021/jf503773u

DO - 10.1021/jf503773u

M3 - Article

VL - 63

SP - 370

EP - 378

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 1

ER -