Evaluation of immune and apoptosis related gene responses using an RNAi approach in vaccinated Penaeus monodon during oral WSSV infection

A.D. Kulkarni; C.M.A. Caipang; V. Kiron; J.H.W.M. Rombout; J.M.O. Fernandes; M. Brinchmann

doi:10.1016/j.margen.2014.05.002

Evaluation of immune and apoptosis related gene responses using an RNAi approach in vaccinated Penaeus monodon during oral WSSV infection

A.D. Kulkarni, C.M.A. Caipang, V. Kiron, J.H.W.M. Rombout, J.M.O. Fernandes, M. Brinchmann

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

In the present study RNA interference was used to elucidate the connection between two endogenous genes [Penaeus monodon Rab7 (PmRab7) or P. monodon inhibitor of apoptosis (PmIAP)], and selected immune/apoptosis-related genes in orally ‘vaccinated’ shrimp after white spot syndrome virus (WSSV) infection. P. monodon were vaccinated by feeding them with formalin inactivated WSSV-coated feed. Thereafter, PmRab7 or PmIAP genes were silenced by injecting the shrimps with their respective dsRNA. The resulting groups of shrimps, Rab7 and IAP, were orally infected with WSSV and the expression of three immune-relevant genes in Rab7 group and five apoptosis-related genes in IAP group was evaluated. In the Rab7 group, PmToll, PmPPAE 2 and Pm penaeidin genes were down-regulated. The IAP-silenced shrimps were characterized by down-regulation of Pm caspase, PmERp57, Pm14-3-3 e, Pm ald, and up-regulation of PmSTAT. Thus, silencing of PmRab7/PmIAP has provided important clues on their relationship with selected immune/apoptosis genes in orally vaccinated P. monodon during WSSV infection.

Original language	English
Pages (from-to)	55-65
Journal	Marine Genomics
Volume	18
Issue number	part A
DOIs	https://doi.org/10.1016/j.margen.2014.05.002
Publication status	Published - 2014

Keywords

spot-syndrome-virus
double-stranded-rna
black tiger shrimp
toll-like receptors
litopenaeus-vannamei
molecular-cloning
innate immunity
fenneropenaeus-chinensis
expression analysis
vibrio-anguillarum

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.margen.2014.05.002

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@article{a5ecbdb002c54a5d8dfe1006bb03046f,

title = "Evaluation of immune and apoptosis related gene responses using an RNAi approach in vaccinated Penaeus monodon during oral WSSV infection",

abstract = "In the present study RNA interference was used to elucidate the connection between two endogenous genes [Penaeus monodon Rab7 (PmRab7) or P. monodon inhibitor of apoptosis (PmIAP)], and selected immune/apoptosis-related genes in orally {\textquoteleft}vaccinated{\textquoteright} shrimp after white spot syndrome virus (WSSV) infection. P. monodon were vaccinated by feeding them with formalin inactivated WSSV-coated feed. Thereafter, PmRab7 or PmIAP genes were silenced by injecting the shrimps with their respective dsRNA. The resulting groups of shrimps, Rab7 and IAP, were orally infected with WSSV and the expression of three immune-relevant genes in Rab7 group and five apoptosis-related genes in IAP group was evaluated. In the Rab7 group, PmToll, PmPPAE 2 and Pm penaeidin genes were down-regulated. The IAP-silenced shrimps were characterized by down-regulation of Pm caspase, PmERp57, Pm14-3-3 e, Pm ald, and up-regulation of PmSTAT. Thus, silencing of PmRab7/PmIAP has provided important clues on their relationship with selected immune/apoptosis genes in orally vaccinated P. monodon during WSSV infection.",

keywords = "spot-syndrome-virus, double-stranded-rna, black tiger shrimp, toll-like receptors, litopenaeus-vannamei, molecular-cloning, innate immunity, fenneropenaeus-chinensis, expression analysis, vibrio-anguillarum",

author = "A.D. Kulkarni and C.M.A. Caipang and V. Kiron and J.H.W.M. Rombout and J.M.O. Fernandes and M. Brinchmann",

year = "2014",

doi = "10.1016/j.margen.2014.05.002",

language = "English",

volume = "18",

pages = "55--65",

journal = "Marine Genomics",

issn = "1874-7787",

publisher = "Elsevier",

number = "part A",

}

TY - JOUR

T1 - Evaluation of immune and apoptosis related gene responses using an RNAi approach in vaccinated Penaeus monodon during oral WSSV infection

AU - Kulkarni, A.D.

AU - Caipang, C.M.A.

AU - Kiron, V.

AU - Rombout, J.H.W.M.

AU - Fernandes, J.M.O.

AU - Brinchmann, M.

PY - 2014

Y1 - 2014

N2 - In the present study RNA interference was used to elucidate the connection between two endogenous genes [Penaeus monodon Rab7 (PmRab7) or P. monodon inhibitor of apoptosis (PmIAP)], and selected immune/apoptosis-related genes in orally ‘vaccinated’ shrimp after white spot syndrome virus (WSSV) infection. P. monodon were vaccinated by feeding them with formalin inactivated WSSV-coated feed. Thereafter, PmRab7 or PmIAP genes were silenced by injecting the shrimps with their respective dsRNA. The resulting groups of shrimps, Rab7 and IAP, were orally infected with WSSV and the expression of three immune-relevant genes in Rab7 group and five apoptosis-related genes in IAP group was evaluated. In the Rab7 group, PmToll, PmPPAE 2 and Pm penaeidin genes were down-regulated. The IAP-silenced shrimps were characterized by down-regulation of Pm caspase, PmERp57, Pm14-3-3 e, Pm ald, and up-regulation of PmSTAT. Thus, silencing of PmRab7/PmIAP has provided important clues on their relationship with selected immune/apoptosis genes in orally vaccinated P. monodon during WSSV infection.

AB - In the present study RNA interference was used to elucidate the connection between two endogenous genes [Penaeus monodon Rab7 (PmRab7) or P. monodon inhibitor of apoptosis (PmIAP)], and selected immune/apoptosis-related genes in orally ‘vaccinated’ shrimp after white spot syndrome virus (WSSV) infection. P. monodon were vaccinated by feeding them with formalin inactivated WSSV-coated feed. Thereafter, PmRab7 or PmIAP genes were silenced by injecting the shrimps with their respective dsRNA. The resulting groups of shrimps, Rab7 and IAP, were orally infected with WSSV and the expression of three immune-relevant genes in Rab7 group and five apoptosis-related genes in IAP group was evaluated. In the Rab7 group, PmToll, PmPPAE 2 and Pm penaeidin genes were down-regulated. The IAP-silenced shrimps were characterized by down-regulation of Pm caspase, PmERp57, Pm14-3-3 e, Pm ald, and up-regulation of PmSTAT. Thus, silencing of PmRab7/PmIAP has provided important clues on their relationship with selected immune/apoptosis genes in orally vaccinated P. monodon during WSSV infection.

KW - spot-syndrome-virus

KW - double-stranded-rna

KW - black tiger shrimp

KW - toll-like receptors

KW - litopenaeus-vannamei

KW - molecular-cloning

KW - innate immunity

KW - fenneropenaeus-chinensis

KW - expression analysis

KW - vibrio-anguillarum

U2 - 10.1016/j.margen.2014.05.002

DO - 10.1016/j.margen.2014.05.002

M3 - Article

SN - 1874-7787

VL - 18

SP - 55

EP - 65

JO - Marine Genomics

JF - Marine Genomics

IS - part A

ER -

Evaluation of immune and apoptosis related gene responses using an RNAi approach in vaccinated Penaeus monodon during oral WSSV infection

Abstract

Keywords

UN SDGs

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