Evaluation of diagnostic tests for the detection of classical swine fever in the field without a gold standard

A. Bouma, J.A. Stegeman, B. Engel, E.P. de Kluijver, A.R.W. Elbers, M.C.M. de Jong

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)

Abstract

Knowledge of the sensitivity of diagnostic tests for infectious diseases under field conditions can be used to design a surveillance program that increases the effectiveness of the control policy. In this study, the sensitivity of tests for the detection of classical swine fever (CSF) virus (CSFV) under field conditions was estimated without knowledge of the true disease status of the animals tested. During the CSF epidemic of 1997-1998 in The Netherlands, tonsil samples from pigs of CSF suspect farms were collected for laboratory diagnosis of CSF. These specimens were tested in a fluorescence antibody test (FAT1) for the presence of CSFV antigen. When at least 1 specimen in a particular sample series from a farm was positive, this farm was declared CSFV infected. Specimens of that series, either FAT1 negative (98) or FAT1 positive (127), were subsequently tested again (FAT2). After that, a suspension was made of the remaining tissue, and this suspension was evaluated with a virus isolation test. In total, 225 tonsil specimens were examined. A statistical model was formulated, and the sensitivity of the 3 tests and the prevalence of positive specimens in the sample were estimated by the method of maximum likelihood. The sensitivity of the FAT1, the test that was used for confirmation of CSFV infection in a pig herd, was approximately 78ø95␌onfidence interval |CI] = 62-92Ž The effectiveness of the selection process of animals on the farm by the veterinarian was estimated to be 77ø64-87Ž The sensitivity of the combination of FAT1 and FAT2 (60€indicates that at least 5 animals should be selected on a CSF-suspect farm to gain a detection probability for CSFV of 99½
Original languageEnglish
Pages (from-to)383-388
JournalJournal of Veterinary Diagnostic Investigation
Volume13
DOIs
Publication statusPublished - 2001

Fingerprint Dive into the research topics of 'Evaluation of diagnostic tests for the detection of classical swine fever in the field without a gold standard'. Together they form a unique fingerprint.

  • Cite this