Evaluation of both targeted and non-targeted cell wall polysaccharides in transgenic potatoes

Jie Hong Huang, Anne Kortstee, Dianka C.T. Dees, Luisa M. Trindade, Richard G.F. Visser, Harry Gruppen, Henk A. Schols*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

In this study, we analyze 31 transgenic lines and their respective untransformed background lines to determine the transgene effects on targeted structures including the pectin components rhamnogalacturonan I (RG-I) and homogalacturonan (HG), neutral side chains (galactan/arabinangalactan), acetylation of pectin, and cellulose level. Modification arising from the pectin backbone- or pectin side chain transgenic lines either increased or decreased the HG:RG-I ratio, side chain length, and methyl esterification of pectin in the tuber cell wall. The pectin esterification transgenic line exhibited only limited side effects. The cellulose level-targeting transgenic lines yielded an unexpectedly high HG:RG-I ratio and longer pectic side chains. These results clearly demonstrate that in effects of a transgene are not restricted to the direct activity of the targeted enzyme but have consequences for the structure of the cell wall matrix. Analysis of whole cell wall structure is therefore necessary to assess the complete effect, direct and indirect, of a transgene.

Original languageEnglish
Pages (from-to)312-321
JournalCarbohydrate Polymers
Volume156
DOIs
Publication statusPublished - 2017

Fingerprint

Polysaccharides
Cells
Esterification
Cellulose
Acetylation
Chain length
Enzymes
Galactans
pectin
rhamnogalacturonan I
polygalacturonic acid

Keywords

  • Galacturonosyltransferase 1
  • Pectin biosynthesis
  • Plant cell wall
  • Rhamnosyltransferase
  • Solanum tuberosum

Cite this

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title = "Evaluation of both targeted and non-targeted cell wall polysaccharides in transgenic potatoes",
abstract = "In this study, we analyze 31 transgenic lines and their respective untransformed background lines to determine the transgene effects on targeted structures including the pectin components rhamnogalacturonan I (RG-I) and homogalacturonan (HG), neutral side chains (galactan/arabinangalactan), acetylation of pectin, and cellulose level. Modification arising from the pectin backbone- or pectin side chain transgenic lines either increased or decreased the HG:RG-I ratio, side chain length, and methyl esterification of pectin in the tuber cell wall. The pectin esterification transgenic line exhibited only limited side effects. The cellulose level-targeting transgenic lines yielded an unexpectedly high HG:RG-I ratio and longer pectic side chains. These results clearly demonstrate that in effects of a transgene are not restricted to the direct activity of the targeted enzyme but have consequences for the structure of the cell wall matrix. Analysis of whole cell wall structure is therefore necessary to assess the complete effect, direct and indirect, of a transgene.",
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author = "Huang, {Jie Hong} and Anne Kortstee and Dees, {Dianka C.T.} and Trindade, {Luisa M.} and Visser, {Richard G.F.} and Harry Gruppen and Schols, {Henk A.}",
year = "2017",
doi = "10.1016/j.carbpol.2016.09.043",
language = "English",
volume = "156",
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journal = "Carbohydrate Polymers",
issn = "0144-8617",
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Evaluation of both targeted and non-targeted cell wall polysaccharides in transgenic potatoes. / Huang, Jie Hong; Kortstee, Anne; Dees, Dianka C.T.; Trindade, Luisa M.; Visser, Richard G.F.; Gruppen, Harry; Schols, Henk A.

In: Carbohydrate Polymers, Vol. 156, 2017, p. 312-321.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Evaluation of both targeted and non-targeted cell wall polysaccharides in transgenic potatoes

AU - Huang, Jie Hong

AU - Kortstee, Anne

AU - Dees, Dianka C.T.

AU - Trindade, Luisa M.

AU - Visser, Richard G.F.

AU - Gruppen, Harry

AU - Schols, Henk A.

PY - 2017

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N2 - In this study, we analyze 31 transgenic lines and their respective untransformed background lines to determine the transgene effects on targeted structures including the pectin components rhamnogalacturonan I (RG-I) and homogalacturonan (HG), neutral side chains (galactan/arabinangalactan), acetylation of pectin, and cellulose level. Modification arising from the pectin backbone- or pectin side chain transgenic lines either increased or decreased the HG:RG-I ratio, side chain length, and methyl esterification of pectin in the tuber cell wall. The pectin esterification transgenic line exhibited only limited side effects. The cellulose level-targeting transgenic lines yielded an unexpectedly high HG:RG-I ratio and longer pectic side chains. These results clearly demonstrate that in effects of a transgene are not restricted to the direct activity of the targeted enzyme but have consequences for the structure of the cell wall matrix. Analysis of whole cell wall structure is therefore necessary to assess the complete effect, direct and indirect, of a transgene.

AB - In this study, we analyze 31 transgenic lines and their respective untransformed background lines to determine the transgene effects on targeted structures including the pectin components rhamnogalacturonan I (RG-I) and homogalacturonan (HG), neutral side chains (galactan/arabinangalactan), acetylation of pectin, and cellulose level. Modification arising from the pectin backbone- or pectin side chain transgenic lines either increased or decreased the HG:RG-I ratio, side chain length, and methyl esterification of pectin in the tuber cell wall. The pectin esterification transgenic line exhibited only limited side effects. The cellulose level-targeting transgenic lines yielded an unexpectedly high HG:RG-I ratio and longer pectic side chains. These results clearly demonstrate that in effects of a transgene are not restricted to the direct activity of the targeted enzyme but have consequences for the structure of the cell wall matrix. Analysis of whole cell wall structure is therefore necessary to assess the complete effect, direct and indirect, of a transgene.

KW - Galacturonosyltransferase 1

KW - Pectin biosynthesis

KW - Plant cell wall

KW - Rhamnosyltransferase

KW - Solanum tuberosum

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