Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples

Ana Palacio-Bielsa*, Pablo López-Soriano, Andreas Bühlmann, Joop van Doorn, Khanh Pham, Miguel A. Cambra, Isabel M. Berruete, Joël F. Pothier, Brion Duffy, Antonio Olmos, María M. López

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    6 Citations (Scopus)

    Abstract

    Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods.

    Original languageEnglish
    Pages (from-to)36-39
    JournalJournal of Microbiological Methods
    Volume112
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Diagnostic assays
    • LAMP
    • Real-time PCR
    • Xanthomonas arboricola pv. pruni

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