Evaluation of a loop-mediated isothermal amplification (LAMP) method for rapid on-site detection of horse meat

Aafke Aartse, Ingrid Scholtens-Toma, Hans J.G. van der A, Monique M. Boersma-Greve, Theo W. Prins, Leen A. van Ginkel, Esther J. Kok, Toine F.H. Bovee

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Detection of horse DNA by loop-mediated isothermal amplification (LAMP) seems one of the most promising methods to meet the criteria of fast, robust, cost efficient, specific, and sensitive on-site detection. In the present study an assessment of the specificity and sensitivity of the LAMP horse screening assay was made and outcomes were compared with the EURL-AP (European Union Reference laboratory for Animal Proteins in feeding stuffs) qPCR method. The specificity was tested with DNA samples from seven other species. The sensitivity of the LAMP assay was subsequently challenged with different percentages of horse DNA in cattle DNA and different percentages of horse meat in cattle meat. Both qPCR and LAMP were able to reliably detect horse DNA or meat below 0.1%, but LAMP was able to do so in less than 30 min. The DNA of other species did not result in a response in the LAMP horse assay. These features show that the LAMP method is fast, specific, and sensitive. Next, 69 processed meat samples were screened for the presence of horse DNA. The results showed that the LAMP horse assay, combined with a simple and fast on-site DNA extraction method, results in similar outcomes as the EURL-AP qPCR method and is thus a promising screening assay to be used outside the laboratory. Only samples that are screened on-site as suspect in the LAMP horse assay, need to be brought to the laboratory for confirmation with the more laborious EURL-AP qPCR reference method.

LanguageEnglish
Pages9-15
JournalFood Control
Volume81
DOIs
Publication statusPublished - 2017

Fingerprint

horse meat
rapid methods
Meat
Horses
horses
DNA
animal proteins
Laboratory Animals
assays
European Union
laboratory animals
methodology
meat
screening
loop-mediated isothermal amplification
Proteins
cattle
sampling
Costs and Cost Analysis
Sensitivity and Specificity

Keywords

  • Horse meat
  • Loop-mediated isothermal amplification (LAMP)
  • On-site detection
  • qPCR
  • Rapid method

Cite this

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title = "Evaluation of a loop-mediated isothermal amplification (LAMP) method for rapid on-site detection of horse meat",
abstract = "Detection of horse DNA by loop-mediated isothermal amplification (LAMP) seems one of the most promising methods to meet the criteria of fast, robust, cost efficient, specific, and sensitive on-site detection. In the present study an assessment of the specificity and sensitivity of the LAMP horse screening assay was made and outcomes were compared with the EURL-AP (European Union Reference laboratory for Animal Proteins in feeding stuffs) qPCR method. The specificity was tested with DNA samples from seven other species. The sensitivity of the LAMP assay was subsequently challenged with different percentages of horse DNA in cattle DNA and different percentages of horse meat in cattle meat. Both qPCR and LAMP were able to reliably detect horse DNA or meat below 0.1{\%}, but LAMP was able to do so in less than 30 min. The DNA of other species did not result in a response in the LAMP horse assay. These features show that the LAMP method is fast, specific, and sensitive. Next, 69 processed meat samples were screened for the presence of horse DNA. The results showed that the LAMP horse assay, combined with a simple and fast on-site DNA extraction method, results in similar outcomes as the EURL-AP qPCR method and is thus a promising screening assay to be used outside the laboratory. Only samples that are screened on-site as suspect in the LAMP horse assay, need to be brought to the laboratory for confirmation with the more laborious EURL-AP qPCR reference method.",
keywords = "Horse meat, Loop-mediated isothermal amplification (LAMP), On-site detection, qPCR, Rapid method",
author = "Aafke Aartse and Ingrid Scholtens-Toma and {van der A}, {Hans J.G.} and Boersma-Greve, {Monique M.} and Prins, {Theo W.} and {van Ginkel}, {Leen A.} and Kok, {Esther J.} and Bovee, {Toine F.H.}",
year = "2017",
doi = "10.1016/j.foodcont.2017.05.025",
language = "English",
volume = "81",
pages = "9--15",
journal = "Food Control",
issn = "0956-7135",
publisher = "Elsevier",

}

Evaluation of a loop-mediated isothermal amplification (LAMP) method for rapid on-site detection of horse meat. / Aartse, Aafke; Scholtens-Toma, Ingrid; van der A, Hans J.G.; Boersma-Greve, Monique M.; Prins, Theo W.; van Ginkel, Leen A.; Kok, Esther J.; Bovee, Toine F.H.

In: Food Control, Vol. 81, 2017, p. 9-15.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Evaluation of a loop-mediated isothermal amplification (LAMP) method for rapid on-site detection of horse meat

AU - Aartse, Aafke

AU - Scholtens-Toma, Ingrid

AU - van der A, Hans J.G.

AU - Boersma-Greve, Monique M.

AU - Prins, Theo W.

AU - van Ginkel, Leen A.

AU - Kok, Esther J.

AU - Bovee, Toine F.H.

PY - 2017

Y1 - 2017

N2 - Detection of horse DNA by loop-mediated isothermal amplification (LAMP) seems one of the most promising methods to meet the criteria of fast, robust, cost efficient, specific, and sensitive on-site detection. In the present study an assessment of the specificity and sensitivity of the LAMP horse screening assay was made and outcomes were compared with the EURL-AP (European Union Reference laboratory for Animal Proteins in feeding stuffs) qPCR method. The specificity was tested with DNA samples from seven other species. The sensitivity of the LAMP assay was subsequently challenged with different percentages of horse DNA in cattle DNA and different percentages of horse meat in cattle meat. Both qPCR and LAMP were able to reliably detect horse DNA or meat below 0.1%, but LAMP was able to do so in less than 30 min. The DNA of other species did not result in a response in the LAMP horse assay. These features show that the LAMP method is fast, specific, and sensitive. Next, 69 processed meat samples were screened for the presence of horse DNA. The results showed that the LAMP horse assay, combined with a simple and fast on-site DNA extraction method, results in similar outcomes as the EURL-AP qPCR method and is thus a promising screening assay to be used outside the laboratory. Only samples that are screened on-site as suspect in the LAMP horse assay, need to be brought to the laboratory for confirmation with the more laborious EURL-AP qPCR reference method.

AB - Detection of horse DNA by loop-mediated isothermal amplification (LAMP) seems one of the most promising methods to meet the criteria of fast, robust, cost efficient, specific, and sensitive on-site detection. In the present study an assessment of the specificity and sensitivity of the LAMP horse screening assay was made and outcomes were compared with the EURL-AP (European Union Reference laboratory for Animal Proteins in feeding stuffs) qPCR method. The specificity was tested with DNA samples from seven other species. The sensitivity of the LAMP assay was subsequently challenged with different percentages of horse DNA in cattle DNA and different percentages of horse meat in cattle meat. Both qPCR and LAMP were able to reliably detect horse DNA or meat below 0.1%, but LAMP was able to do so in less than 30 min. The DNA of other species did not result in a response in the LAMP horse assay. These features show that the LAMP method is fast, specific, and sensitive. Next, 69 processed meat samples were screened for the presence of horse DNA. The results showed that the LAMP horse assay, combined with a simple and fast on-site DNA extraction method, results in similar outcomes as the EURL-AP qPCR method and is thus a promising screening assay to be used outside the laboratory. Only samples that are screened on-site as suspect in the LAMP horse assay, need to be brought to the laboratory for confirmation with the more laborious EURL-AP qPCR reference method.

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KW - On-site detection

KW - qPCR

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JO - Food Control

T2 - Food Control

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SN - 0956-7135

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