Evaluation of a Commercial ELISA for Detection of Ruminant Processed Animal Proteins in Non-Ruminant Processed Animal Proteins

M.G.E.G. Bremer, R.J.C.F. Margry, J.C.H. Vaessen, A.M.H. van Doremalen, J.G.P. van der Palen, R.G.C. van Kaathoven, A.E.M. Kemmers-Voncken, L.W.D. van Raamsdonk

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Abstract

Due to a growing aquaculture industry, demand for high-quality proteins for aquatic feeds is increasing. Non-ruminant processed animal proteins (PAPs) have shown great potential for this purpose. Safe reintroduction of non-ruminant PAPs in aqua feed requires methods that can discriminate ruminant and non-ruminant PAPs at contamination levels at or below 2%. Because the official European Union method lacks species specificity, the performance of MELISA-TEK™ Ruminant, a commercial immunoassay, combined with the MELISA-TEK High Sensitivity Sample Extraction kit was evaluated. Various non-ruminant PAPs spiked with ruminant PAPs (processed at 133, 137, 141, and 145°C) were analyzed. Results showed an overall specificity of 99%, indicating no cross-reaction with non-ruminant PAPs. The sensitivity of the assay strongly depended on both processing temperature and proportion of muscle fibers of the ruminant PAPs. Overall sensitivity of samples with 1 and 2% ruminant PAPs was 92 and 100%, respectively. For ruminant PAPs processed at 133 and 137°C, the sensitivity was 100% for both 1 and 2% ruminant spikes. Overall accuracies were 96 and 99% for 1 and 2% ruminant spikes, respectively. In conclusion, the MELISA-TEK Ruminant assay showed satisfactory results, which makes it a suitable candidate method to enable safe reintroduction of non-ruminant PAPs in aqua feed.
Original languageEnglish
Pages (from-to)552-559
JournalJournal of AOAC International
Volume96
Issue number3
DOIs
Publication statusPublished - 2013

Keywords

  • plant sterilization conditions
  • shrimp litopenaeus-vannamei
  • linked-immunosorbent-assay
  • bone meals
  • classical microscopy
  • rendered meat
  • by-products
  • feed
  • pcr
  • identification

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    VWA Nationaal Plan Diervoeders (WOT-02-004-016)

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    Cite this

    Bremer, M. G. E. G., Margry, R. J. C. F., Vaessen, J. C. H., van Doremalen, A. M. H., van der Palen, J. G. P., van Kaathoven, R. G. C., Kemmers-Voncken, A. E. M., & van Raamsdonk, L. W. D. (2013). Evaluation of a Commercial ELISA for Detection of Ruminant Processed Animal Proteins in Non-Ruminant Processed Animal Proteins. Journal of AOAC International, 96(3), 552-559. https://doi.org/10.5740/jaoacint.11-556