European Population of Pectobacterium punjabense: Genomic Diversity, Tuber Maceration Capacity and a Detection Tool  for This Rarely Occurring Potato Pathogen

Jeremy  Cigna; Angelique Laurent; Malgorzata Waleron; Krzysztof Waleron; Pauline Dewaegeneire; J.M. van der Wolf; Didier Andrivon; Denis Faure; Valerie Helias

doi:10.3390/microorganisms9040781

European Population of Pectobacterium punjabense: Genomic Diversity, Tuber Maceration Capacity and a Detection Tool for This Rarely Occurring Potato Pathogen

Jeremy Cigna^*, Angelique Laurent, Malgorzata Waleron, Krzysztof Waleron, Pauline Dewaegeneire, J.M. van der Wolf, Didier Andrivon, Denis Faure, Valerie Helias^*

^*Corresponding author for this work

Biointeractions and Plant Health

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

Enterobacteria belonging to the Pectobacterium and Dickeya genera are responsible for soft rot and blackleg diseases occurring in many crops around the world. Since 2016, the number of described species has more than doubled. However, some new species, such as Pectobacterium pun‐jabense, are often poorly characterized, and little is known about their genomic and phenotypic variation. Here, we explored several European culture collections and identified seven strains of P.punjabense. All were collected from potato blackleg symptoms, sometimes from a long time ago, i.e., the IFB5596 strain isolated almost 25 years ago. We showed that this species remains rare, with less
than 0.24% of P. punjabense strains identified among pectinolytic bacteria present in the surveyed collections. The analysis of the genomic diversity revealed the non‐clonal character of P. punjabense species. Furthermore, the strains showed aggressiveness differences. Finally, a qPCR Taqman assay
was developed for rapid and specific strain characterization and for use in diagnostic programs.

Original language	English
Article number	781
Journal	Microorganisms
Volume	9
Issue number	4
DOIs	https://doi.org/10.3390/microorganisms9040781
Publication status	Published - 8 Apr 2021

Keywords

soft rot enterobacteria
Pectobacterium punjabense
genomic diversity
detection qPCR TaqMan assay
maceration tuber

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title = "European Population of Pectobacterium punjabense: Genomic Diversity, Tuber Maceration Capacity and a Detection Tool for This Rarely Occurring Potato Pathogen",

abstract = "Enterobacteria belonging to the Pectobacterium and Dickeya genera are responsible for soft rot and blackleg diseases occurring in many crops around the world. Since 2016, the number of described species has more than doubled. However, some new species, such as Pectobacterium pun‐jabense, are often poorly characterized, and little is known about their genomic and phenotypic variation. Here, we explored several European culture collections and identified seven strains of P.punjabense. All were collected from potato blackleg symptoms, sometimes from a long time ago, i.e., the IFB5596 strain isolated almost 25 years ago. We showed that this species remains rare, with lessthan 0.24% of P. punjabense strains identified among pectinolytic bacteria present in the surveyed collections. The analysis of the genomic diversity revealed the non‐clonal character of P. punjabense species. Furthermore, the strains showed aggressiveness differences. Finally, a qPCR Taqman assaywas developed for rapid and specific strain characterization and for use in diagnostic programs.",

keywords = "soft rot enterobacteria, Pectobacterium punjabense, genomic diversity, detection qPCR TaqMan assay, maceration tuber",

author = "Jeremy Cigna and Angelique Laurent and Malgorzata Waleron and Krzysztof Waleron and Pauline Dewaegeneire and {van der Wolf}, J.M. and Didier Andrivon and Denis Faure and Valerie Helias",

year = "2021",

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doi = "10.3390/microorganisms9040781",

language = "English",

volume = "9",

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T1 - European Population of Pectobacterium punjabense: Genomic Diversity, Tuber Maceration Capacity and a Detection Tool for This Rarely Occurring Potato Pathogen

AU - Cigna, Jeremy

AU - Laurent, Angelique

AU - Waleron, Malgorzata

AU - Waleron, Krzysztof

AU - Dewaegeneire, Pauline

AU - van der Wolf, J.M.

AU - Andrivon, Didier

AU - Faure, Denis

AU - Helias, Valerie

PY - 2021/4/8

Y1 - 2021/4/8

N2 - Enterobacteria belonging to the Pectobacterium and Dickeya genera are responsible for soft rot and blackleg diseases occurring in many crops around the world. Since 2016, the number of described species has more than doubled. However, some new species, such as Pectobacterium pun‐jabense, are often poorly characterized, and little is known about their genomic and phenotypic variation. Here, we explored several European culture collections and identified seven strains of P.punjabense. All were collected from potato blackleg symptoms, sometimes from a long time ago, i.e., the IFB5596 strain isolated almost 25 years ago. We showed that this species remains rare, with lessthan 0.24% of P. punjabense strains identified among pectinolytic bacteria present in the surveyed collections. The analysis of the genomic diversity revealed the non‐clonal character of P. punjabense species. Furthermore, the strains showed aggressiveness differences. Finally, a qPCR Taqman assaywas developed for rapid and specific strain characterization and for use in diagnostic programs.

AB - Enterobacteria belonging to the Pectobacterium and Dickeya genera are responsible for soft rot and blackleg diseases occurring in many crops around the world. Since 2016, the number of described species has more than doubled. However, some new species, such as Pectobacterium pun‐jabense, are often poorly characterized, and little is known about their genomic and phenotypic variation. Here, we explored several European culture collections and identified seven strains of P.punjabense. All were collected from potato blackleg symptoms, sometimes from a long time ago, i.e., the IFB5596 strain isolated almost 25 years ago. We showed that this species remains rare, with lessthan 0.24% of P. punjabense strains identified among pectinolytic bacteria present in the surveyed collections. The analysis of the genomic diversity revealed the non‐clonal character of P. punjabense species. Furthermore, the strains showed aggressiveness differences. Finally, a qPCR Taqman assaywas developed for rapid and specific strain characterization and for use in diagnostic programs.

KW - soft rot enterobacteria

KW - Pectobacterium punjabense

KW - genomic diversity

KW - detection qPCR TaqMan assay

KW - maceration tuber

U2 - 10.3390/microorganisms9040781

DO - 10.3390/microorganisms9040781

M3 - Article

SN - 2076-2607

VL - 9

JO - Microorganisms

JF - Microorganisms

IS - 4

M1 - 781

ER -

European Population of Pectobacterium punjabense: Genomic Diversity, Tuber Maceration Capacity and a Detection Tool for This Rarely Occurring Potato Pathogen

Abstract

Keywords

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