European Population of Pectobacterium punjabense: Genomic Diversity, Tuber Maceration Capacity and a Detection Tool for This Rarely Occurring Potato Pathogen

Jeremy Cigna*, Angelique Laurent, Malgorzata Waleron, Krzysztof Waleron, Pauline Dewaegeneire, J.M. van der Wolf, Didier Andrivon, Denis Faure, Valerie Helias

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Enterobacteria belonging to the Pectobacterium and Dickeya genera are responsible for soft rot and blackleg diseases occurring in many crops around the world. Since 2016, the number of described species has more than doubled. However, some new species, such as Pectobacterium pun‐jabense, are often poorly characterized, and little is known about their genomic and phenotypic variation. Here, we explored several European culture collections and identified seven strains of P.punjabense. All were collected from potato blackleg symptoms, sometimes from a long time ago, i.e., the IFB5596 strain isolated almost 25 years ago. We showed that this species remains rare, with less
than 0.24% of P. punjabense strains identified among pectinolytic bacteria present in the surveyed collections. The analysis of the genomic diversity revealed the non‐clonal character of P. punjabense species. Furthermore, the strains showed aggressiveness differences. Finally, a qPCR Taqman assay
was developed for rapid and specific strain characterization and for use in diagnostic programs.
Original languageEnglish
Article number781
JournalMicroorganisms
Volume9
Issue number4
DOIs
Publication statusPublished - 8 Apr 2021

Keywords

  • soft rot enterobacteria
  • Pectobacterium punjabense
  • genomic diversity
  • detection qPCR TaqMan assay
  • maceration tuber

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