EURALLIVEG: Establishment of a European core collection by cryopreservation and virus elimination in garlic

E.R.J. Keller, C.D. Zanke, F.R. Blattner, C. Kik, H. Stavelíková, J. Zámecník, F. Esnault, T. Kotlinska, S. Solberg, V. Miccolis

    Research output: Chapter in Book/Report/Conference proceedingConference paperAcademic

    5 Citations (Scopus)

    Abstract

    A European project named EURALLIVEG (Vegetative Allium, Europe’s Core Collection, safe & sound) was performed under Council Regulation EC 870/2004 from 2007 until 2011. It was coordinated by IPK. The main focus of the project was the development of a European integrated Allium Core collection provided by national collections of Germany, Czech Republic, Poland, Italy, France, and Nordic countries. This collection was designed as a part of the European Genebank Integrated System (AEGIS) of the European Cooperative Program for Plant Genetic Resources (ECPGR). The project consists of five work packages: documentation, molecular screening, cryopreservation, virus elimination, and coordination. Main aim was identification of a core collection of the 200 most important garlic accessions. This collection was cryopreserved using vitrification, which is the most economic way for long term storage. A Cryobanks Network was organized by three project partners. This system ensures safe storage of material by adopting a safety duplicate strategy. The documentation of the collection used passport and characterization data, pictures and other information. In addition genetic fingerprinting with AFLP markers was conducted. In the project, the garlic accessions were introduced into meristem culture for virus elimination. Of the viruses present in garlic, Onion Yellow Dwarf (OYDV) and Leek Yellow Stripe (LYSV) Viruses are most harmful. Therefore, because of the impossibility to do all treatments and tests on all germplasm, it was decided to test the major part of the collection on OYDV and LYSV only. However, a specific part with higher priority (backbone subset) was examined for five viruses. Virus detection used ELISA testing.
    Original languageEnglish
    Title of host publicationProceedings of the VI Internationaal Symposium on Edible Alliaceae, 21-24 May 2012, Fukuoka, Japan
    EditorsT. Wako, M. Shigyo
    PublisherISHS
    Pages319-327
    Volume969
    ISBN (Print)9789066056954
    DOIs
    Publication statusPublished - 2012
    EventVI Internationaal Symposium on Edible Alliaceae, Fukuoka, Japan -
    Duration: 21 May 201224 May 2012

    Conference

    ConferenceVI Internationaal Symposium on Edible Alliaceae, Fukuoka, Japan
    Period21/05/1224/05/12

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