Euphresco Sendo: An international laboratory comparison study of molecular tests for Synchytrium endobioticum detection and identification

Bart van de Vossenberg*, Marcel Westenberg, Ian Adams, Olga Afanasenko, Ani Besheva, Margriet Boerma, James Choiseul, Toos Dekker, Kerstin Flath, Marga van Gent-Pelzer, Kurt Heungens, Anatolii Karelov, Ilona Kibildiene, Jaroslaw Przetakiewicz, Alexandra Schlenzig, Vera Yakovleva, Gerard van Leeuwen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

An international test performance study (TPS) was organised to generate validation data for three molecular Synchytrium endobioticum tests: van den Boogert et al. (European Journal of Plant Pathology 113, 47–57, 2005), and van Gent-Pelzer et al. (European Journal of Plant Pathology, 126, 129-133, 2010) for the detection of S. endobioticum, and the pathotype 1(D1) identification test described by Bonants et al. (European Journal of Plant Pathology, 143, 495-506, 2015). Two TPS rounds were organised focussing on different test matrices, i.e. round 1: warted potato tissue, and round 2: resting spore suspensions. When using the tests for detection and identification of S. endobioticum in warted potato tissue, no significant differences were observed for diagnostic sensitivity, diagnostic specificity, overall accuracy, analytical sensitivity and robustness. When using the tests for detection and identification of S. endobioticum in resting spore suspensions, the van den Boogert and van Gent-Pelzer tests significantly outperform the Bonants test for diagnostic sensitivity and diagnostic specificity. For overall accuracy and analytical sensitivity, the van Gent-Pelzer significantly outperforms the van den Boogert and Bonants tests and is regarded as the test of choice when identifying S. endobioticum from resting spores. Tests regarded fit for purpose for routine testing of wart material and resting spore suspensions are proposed for the update of EPPO st andar d PM7/ 28(1) Synchyt ri um endobioticum.

Original languageEnglish
Pages (from-to)757-766
Number of pages10
JournalEuropean Journal of Plant Pathology
Volume151
Issue number3
DOIs
Publication statusPublished - Jul 2018

Keywords

  • EPPO diagnostic standard
  • Potato wart disease
  • Test performance study inter-laboratory comparison study
  • Validation

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