Eukaryotic diversity in historical soil samples

S.Y. Moon-van der Staay; V.A. Tzeneva; G.W.M. van der Staay; W.M. de Vos; H. Smidt; J.H.P. Hackstein

doi:10.1111/j.1574-6941.2006.00130.x

Eukaryotic diversity in historical soil samples

S.Y. Moon-van der Staay, V.A. Tzeneva, G.W.M. van der Staay, W.M. de Vos, H. Smidt, J.H.P. Hackstein

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The eukaryotic biodiversity in historical air-dried samples of Dutch agricultural soil has been assessed by random sequencing of an 18S rRNA gene library and by denaturing gradient gel electrophoresis. Representatives of nearly all taxa of eukaryotic soil microbes could be identified, demonstrating that it is possible to study eukaryotic microbiota in samples from soil archives that have been stored for more than 30 years at room temperature. In a pilot study, 41 sequences were retrieved that could be assigned to fungi and a variety of aerobic and anaerobic protists such as cercozoans, ciliates, xanthophytes (stramenopiles), heteroloboseans, and amoebozoans. A PCR-denaturing gradient gel electrophoresis analysis of samples collected between 1950 and 1975 revealed significant changes in the composition of the eukaryotic microbiota.

Original language	English
Pages (from-to)	420-428
Journal	FEMS Microbiology Ecology
Volume	57
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-6941.2006.00130.x
Publication status	Published - 2006

Keywords

polymerase chain-reaction
gel-electrophoresis dgge
18s rdna sequences
ribosomal-rna
molecular techniques
anoxic environments
dna
protozoa
ecology
populations

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10.1111/j.1574-6941.2006.00130.x

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title = "Eukaryotic diversity in historical soil samples",

abstract = "The eukaryotic biodiversity in historical air-dried samples of Dutch agricultural soil has been assessed by random sequencing of an 18S rRNA gene library and by denaturing gradient gel electrophoresis. Representatives of nearly all taxa of eukaryotic soil microbes could be identified, demonstrating that it is possible to study eukaryotic microbiota in samples from soil archives that have been stored for more than 30 years at room temperature. In a pilot study, 41 sequences were retrieved that could be assigned to fungi and a variety of aerobic and anaerobic protists such as cercozoans, ciliates, xanthophytes (stramenopiles), heteroloboseans, and amoebozoans. A PCR-denaturing gradient gel electrophoresis analysis of samples collected between 1950 and 1975 revealed significant changes in the composition of the eukaryotic microbiota.",

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doi = "10.1111/j.1574-6941.2006.00130.x",

language = "English",

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AU - Moon-van der Staay, S.Y.

AU - Tzeneva, V.A.

AU - van der Staay, G.W.M.

AU - de Vos, W.M.

AU - Smidt, H.

AU - Hackstein, J.H.P.

PY - 2006

Y1 - 2006

N2 - The eukaryotic biodiversity in historical air-dried samples of Dutch agricultural soil has been assessed by random sequencing of an 18S rRNA gene library and by denaturing gradient gel electrophoresis. Representatives of nearly all taxa of eukaryotic soil microbes could be identified, demonstrating that it is possible to study eukaryotic microbiota in samples from soil archives that have been stored for more than 30 years at room temperature. In a pilot study, 41 sequences were retrieved that could be assigned to fungi and a variety of aerobic and anaerobic protists such as cercozoans, ciliates, xanthophytes (stramenopiles), heteroloboseans, and amoebozoans. A PCR-denaturing gradient gel electrophoresis analysis of samples collected between 1950 and 1975 revealed significant changes in the composition of the eukaryotic microbiota.

AB - The eukaryotic biodiversity in historical air-dried samples of Dutch agricultural soil has been assessed by random sequencing of an 18S rRNA gene library and by denaturing gradient gel electrophoresis. Representatives of nearly all taxa of eukaryotic soil microbes could be identified, demonstrating that it is possible to study eukaryotic microbiota in samples from soil archives that have been stored for more than 30 years at room temperature. In a pilot study, 41 sequences were retrieved that could be assigned to fungi and a variety of aerobic and anaerobic protists such as cercozoans, ciliates, xanthophytes (stramenopiles), heteroloboseans, and amoebozoans. A PCR-denaturing gradient gel electrophoresis analysis of samples collected between 1950 and 1975 revealed significant changes in the composition of the eukaryotic microbiota.

KW - polymerase chain-reaction

KW - gel-electrophoresis dgge

KW - 18s rdna sequences

KW - ribosomal-rna

KW - molecular techniques

KW - anoxic environments

KW - dna

KW - protozoa

KW - ecology

KW - populations

U2 - 10.1111/j.1574-6941.2006.00130.x

DO - 10.1111/j.1574-6941.2006.00130.x

M3 - Article

SN - 0168-6496

VL - 57

SP - 420

EP - 428

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

IS - 3

ER -

Eukaryotic diversity in historical soil samples

Abstract

Keywords

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