Ethyl acetate production by the elusive alcohol acetyltransferase from yeast

Alex Kruis*, Mark Levisson, Astrid E. Mars, Max van der Ploeg, Fernando Garcés Daza, Valeria Ellena, Servé W.M. Kengen, John van der Oost, Ruud A. Weusthuis

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

37 Citations (Scopus)

Abstract

Ethyl acetate is an industrially relevant ester that is currently produced exclusively through unsustainable processes. Many yeasts are able to produce ethyl acetate, but the main responsible enzyme has remained elusive, hampering the engineering of novel production strains. Here we describe the discovery of a new enzyme (Eat1) from the yeast Wickerhamomyces anomalus that resulted in high ethyl acetate production when expressed in Saccharomyces cerevisiae and Escherichia coli. Purified Eat1 showed alcohol acetyltransferase activity with ethanol and acetyl-CoA. Homologs of eat1 are responsible for most ethyl acetate synthesis in known ethyl acetate-producing yeasts, including S. cerevisiae, and are only distantly related to known alcohol acetyltransferases. Eat1 is therefore proposed to compose a novel alcohol acetyltransferase family within the α/β hydrolase superfamily. The discovery of this novel enzyme family is a crucial step towards the development of biobased ethyl acetate production and will also help in selecting improved S. cerevisiae brewing strains.
Original languageEnglish
Pages (from-to)92-101
JournalMetabolic Engineering
Volume41
DOIs
Publication statusPublished - 2017

Keywords

  • Alcohol acetyltransferase
  • Escherichia coli
  • Ethyl acetate
  • Saccharomyces cerevisiae
  • yeast
  • α/β hydrolase

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