Estimation of variation in concentration, phosphorylation and genetic polymorphism of milk proteins using capillary zone electrophoresis

J.M.L. Heck, N.J. Olieman, A. Schennink, H.J.F. van Valenberg, M.H.P.W. Visker, R.C.R. Meuldijk, A.C.M. van Hooijdonk

Research output: Contribution to journalArticleAcademicpeer-review

64 Citations (Scopus)

Abstract

Capillary zone electrophoresis (CZE) was tested for reproducibility and was shown to be a suitable method for estimating the relative concentration of ¿-lactalbumin, ß-lactoglobulin, ¿S2-casein (¿S2-CN), ¿S1-CN, ¿-CN, and ß-CN in milk. This study showed how the method can also be used to determine the relative concentration of two different phosphorylation states of ¿S1-CN (¿S1-CN-8P and ¿S1-CN-9P) and three of ¿S2-CN (¿S2-CN-10P, ¿S2-CN-11P and ¿S2-CN-12P). Furthermore, with CZE it was possible to determine most common genetic variants including the ¿-CN E variant that has not been identified by CZE before. Analyses of milk samples of 1948 Dutch Holstein-Friesian cows showed large variation in both the relative protein concentration and the relative concentration of different phosphorylation states for all milk proteins studied. Correlations between different phosphorylation states of ¿S1- and ¿S2-CN indicated that the relative concentration of specific phosphorylation states of ¿S1- and ¿S2-CN were related to each other.
Original languageEnglish
Pages (from-to)548-555
JournalInternational Dairy Journal
Volume18
Issue number5
DOIs
Publication statusPublished - 2008

Keywords

  • performance liquid-chromatography
  • bovine-milk
  • quantitative-analysis
  • kappa-casein
  • products
  • cows
  • conversion
  • genotypes
  • peptides

Fingerprint Dive into the research topics of 'Estimation of variation in concentration, phosphorylation and genetic polymorphism of milk proteins using capillary zone electrophoresis'. Together they form a unique fingerprint.

Cite this