Estimation of the transmission of foot-and-mouth disease virus from infected sheep to cattle

C. Bravo De Rueda, M.C.M. de Jong, P.L. Eble, A. Dekker

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

The quantitative role of sheep in the transmission of foot-and-mouth disease virus (FMDV) is not well known. To estimate the role of sheep in the transmission of FMDV, a direct contact transmission experiment with 10 groups of animals each consisting of 2 infected lambs and 1 contact calf was performed. Secretions and excretions (oral swabs, blood, urine, faeces and probang samples) from all animals were tested for the presence of FMDV by virus isolation (VI) and/or RT-PCR. Serum was tested for the presence of antibodies against FMDV. To estimate FMDV transmission, the VI, RT-PCR and serology results were used. The partial reproduction ratio R0 p i.e. the average number of new infections caused by one infected sheep introduced into a population of susceptible cattle, was estimated using either data of the whole infection chain of the experimental epidemics (the transient state method) or the final sizes of the experimental epidemics (the final size method). Using the transient state method, R0 p was estimated as 1.0 (95% CI 0.2 - 6.0) using virus isolation results and 1.4 (95% CI 0.3 - 8.0) using RT-PCR results. Using the final size method, R0 p was estimated as 0.9 (95% CI 0.2 - 3.0). Finally, R0 p was compared to the R0’s obtained in previous transmission studies with sheep or cattle only. This comparison showed that the infectivity of sheep is lower than that of cattle and that sheep and cattle are similarly susceptible to FMD. These results indicate that in a mixed population of sheep and cattle, sheep play a more limited role in the transmission of FMDV than cattle.
Original languageEnglish
Article number58
Number of pages11
JournalVeterinary Research
Volume45
DOIs
Publication statusPublished - 2014

Fingerprint

Foot-and-Mouth Disease Virus
Foot-and-mouth disease virus
Sheep
sheep
cattle
reverse transcriptase polymerase chain reaction
Viruses
Polymerase Chain Reaction
viruses
virus transmission
foot-and-mouth disease
at-risk population
Serology
direct contact
blood serum
Infection
methodology
Feces
infection
Population

Keywords

  • between-pen transmission
  • basic reproduction ratio
  • swine-fever virus
  • emergency vaccination
  • subclinical infection
  • within-pen
  • pigs
  • quantification
  • excretion
  • epidemic

Cite this

@article{12a04c8e4b6e4f348d6d0c96de219c88,
title = "Estimation of the transmission of foot-and-mouth disease virus from infected sheep to cattle",
abstract = "The quantitative role of sheep in the transmission of foot-and-mouth disease virus (FMDV) is not well known. To estimate the role of sheep in the transmission of FMDV, a direct contact transmission experiment with 10 groups of animals each consisting of 2 infected lambs and 1 contact calf was performed. Secretions and excretions (oral swabs, blood, urine, faeces and probang samples) from all animals were tested for the presence of FMDV by virus isolation (VI) and/or RT-PCR. Serum was tested for the presence of antibodies against FMDV. To estimate FMDV transmission, the VI, RT-PCR and serology results were used. The partial reproduction ratio R0 p i.e. the average number of new infections caused by one infected sheep introduced into a population of susceptible cattle, was estimated using either data of the whole infection chain of the experimental epidemics (the transient state method) or the final sizes of the experimental epidemics (the final size method). Using the transient state method, R0 p was estimated as 1.0 (95{\%} CI 0.2 - 6.0) using virus isolation results and 1.4 (95{\%} CI 0.3 - 8.0) using RT-PCR results. Using the final size method, R0 p was estimated as 0.9 (95{\%} CI 0.2 - 3.0). Finally, R0 p was compared to the R0’s obtained in previous transmission studies with sheep or cattle only. This comparison showed that the infectivity of sheep is lower than that of cattle and that sheep and cattle are similarly susceptible to FMD. These results indicate that in a mixed population of sheep and cattle, sheep play a more limited role in the transmission of FMDV than cattle.",
keywords = "between-pen transmission, basic reproduction ratio, swine-fever virus, emergency vaccination, subclinical infection, within-pen, pigs, quantification, excretion, epidemic",
author = "{Bravo De Rueda}, C. and {de Jong}, M.C.M. and P.L. Eble and A. Dekker",
year = "2014",
doi = "10.1186/1297-9716-45-58",
language = "English",
volume = "45",
journal = "Veterinary Research",
issn = "0928-4249",
publisher = "Springer Verlag",

}

Estimation of the transmission of foot-and-mouth disease virus from infected sheep to cattle. / Bravo De Rueda, C.; de Jong, M.C.M.; Eble, P.L.; Dekker, A.

In: Veterinary Research, Vol. 45, 58, 2014.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Estimation of the transmission of foot-and-mouth disease virus from infected sheep to cattle

AU - Bravo De Rueda, C.

AU - de Jong, M.C.M.

AU - Eble, P.L.

AU - Dekker, A.

PY - 2014

Y1 - 2014

N2 - The quantitative role of sheep in the transmission of foot-and-mouth disease virus (FMDV) is not well known. To estimate the role of sheep in the transmission of FMDV, a direct contact transmission experiment with 10 groups of animals each consisting of 2 infected lambs and 1 contact calf was performed. Secretions and excretions (oral swabs, blood, urine, faeces and probang samples) from all animals were tested for the presence of FMDV by virus isolation (VI) and/or RT-PCR. Serum was tested for the presence of antibodies against FMDV. To estimate FMDV transmission, the VI, RT-PCR and serology results were used. The partial reproduction ratio R0 p i.e. the average number of new infections caused by one infected sheep introduced into a population of susceptible cattle, was estimated using either data of the whole infection chain of the experimental epidemics (the transient state method) or the final sizes of the experimental epidemics (the final size method). Using the transient state method, R0 p was estimated as 1.0 (95% CI 0.2 - 6.0) using virus isolation results and 1.4 (95% CI 0.3 - 8.0) using RT-PCR results. Using the final size method, R0 p was estimated as 0.9 (95% CI 0.2 - 3.0). Finally, R0 p was compared to the R0’s obtained in previous transmission studies with sheep or cattle only. This comparison showed that the infectivity of sheep is lower than that of cattle and that sheep and cattle are similarly susceptible to FMD. These results indicate that in a mixed population of sheep and cattle, sheep play a more limited role in the transmission of FMDV than cattle.

AB - The quantitative role of sheep in the transmission of foot-and-mouth disease virus (FMDV) is not well known. To estimate the role of sheep in the transmission of FMDV, a direct contact transmission experiment with 10 groups of animals each consisting of 2 infected lambs and 1 contact calf was performed. Secretions and excretions (oral swabs, blood, urine, faeces and probang samples) from all animals were tested for the presence of FMDV by virus isolation (VI) and/or RT-PCR. Serum was tested for the presence of antibodies against FMDV. To estimate FMDV transmission, the VI, RT-PCR and serology results were used. The partial reproduction ratio R0 p i.e. the average number of new infections caused by one infected sheep introduced into a population of susceptible cattle, was estimated using either data of the whole infection chain of the experimental epidemics (the transient state method) or the final sizes of the experimental epidemics (the final size method). Using the transient state method, R0 p was estimated as 1.0 (95% CI 0.2 - 6.0) using virus isolation results and 1.4 (95% CI 0.3 - 8.0) using RT-PCR results. Using the final size method, R0 p was estimated as 0.9 (95% CI 0.2 - 3.0). Finally, R0 p was compared to the R0’s obtained in previous transmission studies with sheep or cattle only. This comparison showed that the infectivity of sheep is lower than that of cattle and that sheep and cattle are similarly susceptible to FMD. These results indicate that in a mixed population of sheep and cattle, sheep play a more limited role in the transmission of FMDV than cattle.

KW - between-pen transmission

KW - basic reproduction ratio

KW - swine-fever virus

KW - emergency vaccination

KW - subclinical infection

KW - within-pen

KW - pigs

KW - quantification

KW - excretion

KW - epidemic

U2 - 10.1186/1297-9716-45-58

DO - 10.1186/1297-9716-45-58

M3 - Article

VL - 45

JO - Veterinary Research

JF - Veterinary Research

SN - 0928-4249

M1 - 58

ER -