Estimation of hepatitis E virus (HEV) pig seroprevalence using Elisa and Western blot and comparison between human and pig HEV sequences in Belgium.

D. Thiry, A. Mauroy, C. Saegerman, I. Thomas, M. Wautier, C. Miry, G. Czaplicki, D. Berkvens, N. Praet, W.H.M. van der Poel, R. Cariolet, B. Brochier, E. Thiry

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Abstract

Zoonotic transmission of hepatitis E virus (HEV) is of special concern, particularly in high income countries were waterborne infections are less frequent than in developing countries. High HEV seroprevalences can be found in European pig populations. The aims of this study were to obtain prevalence data on HEV infection in swine in Belgium and to phylogenetically compare Belgian human HEV sequences with those obtained from swine. An ELISA screening prevalence of 73% (95% CI 68.8–77.5) was determined in Belgian pigs and a part of the results were re-evaluated by Western blot (WB). A receiver operating characteristic curve analysis was performed and scenarios varying the ELISA specificity relative to WB were analysed. The seroprevalences estimated by the different scenarios ranged between 69 and 81% and are in agreement with the high exposure of the European pig population to HEV. Pig HEV sequences were genetically compared to those detected in humans in Belgium and a predominance of genotype 3 subtype f was shown in both swine and humans. The high HEV seroprevalence in swine and the close phylogenetic relationships between pig and human HEV sequences further support the risk for zoonotic transmission of HEV between humans and pigs.
Original languageEnglish
Pages (from-to)407-414
JournalVeterinary Microbiology
Volume172
Issue number3-4
DOIs
Publication statusPublished - 2014

Fingerprint

Hepatitis E virus
Belgium
Seroepidemiologic Studies
seroprevalence
Western blotting
Swine
Western Blotting
swine
Zoonoses
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Virus Diseases
ROC Curve
infection
Population
Developing Countries
developing countries
income
Genotype

Keywords

  • antibodies
  • swine
  • transmission
  • infection
  • netherlands
  • populations
  • prevalence
  • france
  • meat
  • farm

Cite this

Thiry, D. ; Mauroy, A. ; Saegerman, C. ; Thomas, I. ; Wautier, M. ; Miry, C. ; Czaplicki, G. ; Berkvens, D. ; Praet, N. ; van der Poel, W.H.M. ; Cariolet, R. ; Brochier, B. ; Thiry, E. / Estimation of hepatitis E virus (HEV) pig seroprevalence using Elisa and Western blot and comparison between human and pig HEV sequences in Belgium. In: Veterinary Microbiology. 2014 ; Vol. 172, No. 3-4. pp. 407-414.
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title = "Estimation of hepatitis E virus (HEV) pig seroprevalence using Elisa and Western blot and comparison between human and pig HEV sequences in Belgium.",
abstract = "Zoonotic transmission of hepatitis E virus (HEV) is of special concern, particularly in high income countries were waterborne infections are less frequent than in developing countries. High HEV seroprevalences can be found in European pig populations. The aims of this study were to obtain prevalence data on HEV infection in swine in Belgium and to phylogenetically compare Belgian human HEV sequences with those obtained from swine. An ELISA screening prevalence of 73{\%} (95{\%} CI 68.8–77.5) was determined in Belgian pigs and a part of the results were re-evaluated by Western blot (WB). A receiver operating characteristic curve analysis was performed and scenarios varying the ELISA specificity relative to WB were analysed. The seroprevalences estimated by the different scenarios ranged between 69 and 81{\%} and are in agreement with the high exposure of the European pig population to HEV. Pig HEV sequences were genetically compared to those detected in humans in Belgium and a predominance of genotype 3 subtype f was shown in both swine and humans. The high HEV seroprevalence in swine and the close phylogenetic relationships between pig and human HEV sequences further support the risk for zoonotic transmission of HEV between humans and pigs.",
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author = "D. Thiry and A. Mauroy and C. Saegerman and I. Thomas and M. Wautier and C. Miry and G. Czaplicki and D. Berkvens and N. Praet and {van der Poel}, W.H.M. and R. Cariolet and B. Brochier and E. Thiry",
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Thiry, D, Mauroy, A, Saegerman, C, Thomas, I, Wautier, M, Miry, C, Czaplicki, G, Berkvens, D, Praet, N, van der Poel, WHM, Cariolet, R, Brochier, B & Thiry, E 2014, 'Estimation of hepatitis E virus (HEV) pig seroprevalence using Elisa and Western blot and comparison between human and pig HEV sequences in Belgium.', Veterinary Microbiology, vol. 172, no. 3-4, pp. 407-414. https://doi.org/10.1016/j.vetmic.2014.06.004

Estimation of hepatitis E virus (HEV) pig seroprevalence using Elisa and Western blot and comparison between human and pig HEV sequences in Belgium. / Thiry, D.; Mauroy, A.; Saegerman, C.; Thomas, I.; Wautier, M.; Miry, C.; Czaplicki, G.; Berkvens, D.; Praet, N.; van der Poel, W.H.M.; Cariolet, R.; Brochier, B.; Thiry, E.

In: Veterinary Microbiology, Vol. 172, No. 3-4, 2014, p. 407-414.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Estimation of hepatitis E virus (HEV) pig seroprevalence using Elisa and Western blot and comparison between human and pig HEV sequences in Belgium.

AU - Thiry, D.

AU - Mauroy, A.

AU - Saegerman, C.

AU - Thomas, I.

AU - Wautier, M.

AU - Miry, C.

AU - Czaplicki, G.

AU - Berkvens, D.

AU - Praet, N.

AU - van der Poel, W.H.M.

AU - Cariolet, R.

AU - Brochier, B.

AU - Thiry, E.

PY - 2014

Y1 - 2014

N2 - Zoonotic transmission of hepatitis E virus (HEV) is of special concern, particularly in high income countries were waterborne infections are less frequent than in developing countries. High HEV seroprevalences can be found in European pig populations. The aims of this study were to obtain prevalence data on HEV infection in swine in Belgium and to phylogenetically compare Belgian human HEV sequences with those obtained from swine. An ELISA screening prevalence of 73% (95% CI 68.8–77.5) was determined in Belgian pigs and a part of the results were re-evaluated by Western blot (WB). A receiver operating characteristic curve analysis was performed and scenarios varying the ELISA specificity relative to WB were analysed. The seroprevalences estimated by the different scenarios ranged between 69 and 81% and are in agreement with the high exposure of the European pig population to HEV. Pig HEV sequences were genetically compared to those detected in humans in Belgium and a predominance of genotype 3 subtype f was shown in both swine and humans. The high HEV seroprevalence in swine and the close phylogenetic relationships between pig and human HEV sequences further support the risk for zoonotic transmission of HEV between humans and pigs.

AB - Zoonotic transmission of hepatitis E virus (HEV) is of special concern, particularly in high income countries were waterborne infections are less frequent than in developing countries. High HEV seroprevalences can be found in European pig populations. The aims of this study were to obtain prevalence data on HEV infection in swine in Belgium and to phylogenetically compare Belgian human HEV sequences with those obtained from swine. An ELISA screening prevalence of 73% (95% CI 68.8–77.5) was determined in Belgian pigs and a part of the results were re-evaluated by Western blot (WB). A receiver operating characteristic curve analysis was performed and scenarios varying the ELISA specificity relative to WB were analysed. The seroprevalences estimated by the different scenarios ranged between 69 and 81% and are in agreement with the high exposure of the European pig population to HEV. Pig HEV sequences were genetically compared to those detected in humans in Belgium and a predominance of genotype 3 subtype f was shown in both swine and humans. The high HEV seroprevalence in swine and the close phylogenetic relationships between pig and human HEV sequences further support the risk for zoonotic transmission of HEV between humans and pigs.

KW - antibodies

KW - swine

KW - transmission

KW - infection

KW - netherlands

KW - populations

KW - prevalence

KW - france

KW - meat

KW - farm

U2 - 10.1016/j.vetmic.2014.06.004

DO - 10.1016/j.vetmic.2014.06.004

M3 - Article

VL - 172

SP - 407

EP - 414

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -